Diuretic renal scintigraphy is commonly used in human medicine to differentiate obstructive from nonobstructive pyelectasia. In order to determine normal parameters, 99mTc-DTPA renal scintigraphy was performed twice in 20 healthy adult Mongrel dogs. Each dog was injected with either 3.0 mg/kg furosemide or an equivalent volume of saline 4.5 min following injection of the radiopharmaceutical. The following parameters were evaluated: (1) global and individual glomerular filtration rate (GFR); (2) time of peak (TOP) of the time–activity curve (TAC); (3) shape of the TAC; (4) individual kidney excretion half-time (T1/2); (5) proportion of maximum activity measured at end of study (8 min); (6) time of onset; and (7) duration of the effect of the diuretic. Most TAC in the diuretic renography group showed a steep drop in the curve following administration of the diuretic compared with a gradual slope in the saline control group. There was a statistically significant difference in T1/2 between the control renograms (median 10.25 min, range 4.41–18.07 min) and the diuretic renograms (median 4.16 min, range 3.62–5.90 min). There was a statistically significant difference in percentage maximum activity between the control renograms (median 65.25%, range 48.27–93.68%) and the diuretic renograms (median 48.54%, range 35.64–58.76%). Median time of onset of the diuretic effect was 1.10 min (0.20–2.40 min), and median duration was 0.83 min (0.30–2.35 min).