Original article

Colonisation of soft lining materials by micro-organisms

  1. Sabrina Pavan1,
  2. Paulo Henrique Dos Santos2,
  3. João Neudenir Arioli Filho1,
  4. Denise Madalena Palomare Spolidorio3

Article first published online: 22 JUN 2009

DOI: 10.1111/j.1741-2358.2009.00300.x

Issue

Gerodontology

Gerodontology

Volume 27, Issue 3, pages 211–216, September 2010

Additional Information

How to Cite

Pavan, S., Dos Santos, P. H., Filho, J. N. A. and Spolidorio, D. M. P. (2010), Colonisation of soft lining materials by micro-organisms. Gerodontology, 27: 211–216. doi: 10.1111/j.1741-2358.2009.00300.x

Author Information

  1. 1

    Department of Dental Materials and Prosthodontics, São Paulo State University, Araraquara School of Dentistry, Unesp, Brazil

  2. 2

    Department of Dental Materials and Prosthodontics, São Paulo State University, Aracatuba School of Dentistry, Unesp, Brazil

  3. 3

    Department of Physiology and Pathology, São Paulo State University, Araraquara Dental School, Araraquara, São Paulo, Brazil

*Sabrina Pavan, Department of Dental Materials and Prosthodontics, São Paulo State University, Araraquara School of Dentistry, Unesp, Brazil. Tel.: +55 18 3625 5728 Fax: +55 18 3636 3245 E-mail: sabrinapavan@ig.com.br

Publication History

  1. Issue published online: 23 AUG 2010
  2. Article first published online: 22 JUN 2009
  3. Accepted 27 February 2009

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Keywords:

  • soft lining materials;
  • micro-organisms;
  • surface roughness;
  • colonisation

doi:10.1111/j.1741-2358.2009.00300.x Colonisation of soft lining materials by micro-organisms

Objective:  This study evaluated the in vitro adherence of pathogenic micro-organisms, Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa, to soft lining materials and their inhibitory effect on these micro-organisms.

Materials and Methods:  To measure adherence, specimens of Molloplast B and Ufi Gel P were inoculated [107 colony-forming units per millimetre (cfu/ml)] with TSB media containing the micro-organisms. To determine the number of micro-organisms in the 10−2–10−5 dilutions, 25 μl of the suspension were transferred to plates of selective media. Colony counts of each specimen were quantified (cfu/ml). The surface roughness was measured with a perfilometer to assess the relationship between the adherence of micro-organisms and surface roughness of each material. For the inhibition test, specimens of materials were placed in agar plates inoculated individually with the micro-organisms. After 48 h, the inhibition zones around the specimens were measured.

Results:  None of the materials exhibited inhibition zones. The number of cfu/ml of S. aureus and P. aeruginosa were significantly greater than C. albicans for both materials. The Ufi Gel P exhibited greater adherence of C. albicans than Molloplast B. No correlation was observed between the adherence of micro-organisms and surface roughness.

Conclusion:  The surface roughness of the materials is not the only factor governing micro-organism adherence.

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