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Biological evaluation of the bone healing process after application of two potentially osteogenic proteins: an animal experimental model

Authors

  • João P. M. Issa,

    1. Department of Morphology, Physiology and Stomatology, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Helton L. A. Defino,

    1. Department of Biomechanics, Rehabilitation and Medicine of the Locomotor Apparatus, School of Medicine at Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Walter Sebald,

    1. Theodor-Boveri-Institut für Biowissenschaften, Am Hubland, Würzburg University, Würzburg, Germany
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  • Joaquim Coutinho-Netto,

    1. Department of Immunology and Biochemistry, School of Medicine at Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Mamie M. Iyomasa,

    1. Department of Morphology, Physiology and Stomatology, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Antonio C. Shimano,

    1. Department of Biomechanics, Rehabilitation and Medicine of the Locomotor Apparatus, School of Medicine at Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Maria V. L. B. Bentley,

    1. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil
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  • Dimitrius L. Pitol

    1. Department of Morphology, Physiology and Stomatology, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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João Paulo Mardegan Issa, Professor, Department of Morphology, Physiology and Stomatology, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Tel.: 551636020283
Fax: 551636330999
E-mail: jpmissa@forp.usp.br

Abstract

doi: 10.1111/j.1741-2358.2011.00526.x
Biological evaluation of the bone healing process after application of two potentially osteogenic proteins: an animal experimental model

Objective:  The aim of this work was to analyse qualitatively and quantitatively the newly formed bone after insertion of rhBMP-2 and protein extracted from Hevea brasiliensis (P-1), associated or not with a carrier in critical bone defects created in Wistar rat calvarial bone, using histological and histomorphometrical analyses.

Materials and methods:  Eighty-four male Wistar rats were used, divided into two groups, according to the period of time until the sacrifice (2 and 6 weeks). Each one of these groups was subdivided into six groups with seven animals each, according to the treatments: (1) 5 μg of pure rhBMP-2, (2) 5 μg of rhBMP-2/monoolein gel, (3) pure monoolein gel, (4) 5 μg of pure P-1, (5) 5 μg of P-1/monoolein gel and (6) critical bone defect controls. The animals were euthanised and the calvarial bone tissue removed for histological and histomorphometrical analyses.

Result and conclusion:  The results showed an improvement in the bone healing process using the rhBMP-2 protein, associated or not with a material carrier in relation to the other groups, and this process demonstrated to be time dependent.

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