Efficacy of Fungiflora Y staining for the diagnosis of oral erythematous candidiasis

Authors

  • Mariko R. Okamoto,

    1. Department of Clinical Pathophysiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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  • Miho Kamoi,

    1. Department of Clinical Pathophysiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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  • Shigeo Yamachika,

    1. Department of Clinical Pathophysiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
    2. Department of Oral and Maxillofacial Surgery, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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  • Akihisa Tsurumoto,

    1. Department of Community Dentistry, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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  • Takahiro Imamura,

    1. Department of Clinical Pathophysiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
    2. Department of Oral and Maxillofacial Surgery, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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  • Ken Yamamoto,

    1. Department of Clinical Pathophysiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
    2. Department of Geriatric Dentistry, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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  • Shinichi Kadomatsu,

    1. Department of Clinical Pathophysiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
    2. Department of Geriatric Dentistry, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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  • Ichiro Saito,

    1. Department of Clinical Pathophysiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
    2. Department of Pathology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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  • Nobuko Maeda,

    1. Department of Oral Microbiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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  • Yoichi Nakagawa

    1. Department of Clinical Pathophysiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
    2. Department of Oral and Maxillofacial Surgery, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan
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Yoichi Nakagawa, Department of Oral and Maxillofacial Surgery, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
Fax: +81-45-573-9599
E-mail: nakagawa-y@tsurumi-u.ac.jp

Abstract

doi: 10.1111/j.1741-2358.2012.00668.x

Efficacy of Fungiflora Y staining for the diagnosis of oral erythematous candidiasis

Objective:  The purpose of this study was to investigate the efficacy of Fungiflora Y staining (fluorescent stain) for the diagnosis of erythematous candidiasis.

Subjects and methods:  This study enrolled 48 patients who were diagnosed with erythematous candidiasis and who underwent fungal culture and microscopic examination of a smear specimen stained with CytoQuick (modification of the Giemsa stain) and Fungiflora Y. Fungiflora Y staining was observed using a portable fluorescent microscope (CyScope®). The sensitivity, specificity and positive and negative predictive values were calculated using fungal culture as the gold standard test. Accuracy was calculated, and the difference between the CytoQuick and Fungiflora Y groups was examined using contingency tables and the chi-square test.

Results:  The sensitivity and specificity of the CytoQuick stain was 0.51 and 0.91, respectively; the positive predictive value was 0.95, and the negative predictive value was 0.36. The sensitivity and specificity of the Fungiflora Y stain was 0.84 and 1.0, respectively; the positive predictive value was 1.00, and the negative predictive value was 0.65. The accuracy of Fungiflora Y (0.88) was superior to that of CytoQuick (0.60) (p = 0.0052).

Conclusions:  Microscopic examinations of smear specimens using a combination of Fungiflora Y staining and the CyScope® portable fluorescent microscope was found to be useful for the diagnosis of oral erythematous candidiasis.

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