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Candida albicans adherence to an acrylic resin modified by experimental photopolymerised coatings: an in vitro study

Authors

  • Andrea Azevedo Lazarin,

    1. Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP – Univ Estadual Paulista, São Paulo, CEP, Brazil
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  • Camila Andrade Zamperini,

    1. Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP – Univ Estadual Paulista, São Paulo, CEP, Brazil
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  • Carlos Eduardo Vergani,

    1. Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP – Univ Estadual Paulista, São Paulo, CEP, Brazil
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  • Amanda Fucci Wady,

    1. Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP – Univ Estadual Paulista, São Paulo, CEP, Brazil
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  • Eunice Teresinha Giampaolo,

    1. Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP – Univ Estadual Paulista, São Paulo, CEP, Brazil
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  • Ana Lucia Machado

    Corresponding author
    1. Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP – Univ Estadual Paulista, São Paulo, CEP, Brazil
    • Correspondence to: Prof. Ana L. Machado, Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP – Univ. Estadual Paulista, Araraquara, São Paulo, Rua Humaitá nº 1680, CEP 14.801-903, Brazil.

      Tel.: +55 16 33016543

      Fax: +55 16 33016406

      E-mail: cucci@foar.unesp.br

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Abstract

Purpose

This study evaluated whether photopolymerised coatings containing zwitterion or hydrophilic monomers would reduce the adhesion of Candida albicans to an acrylic resin.

Materials and methods

Disc-shaped samples (n = 468) were fabricated with rough or smooth surfaces. The samples did not receive any surface treatment (control) or were coated with one of the following experimental coatings (2-hydroxyethyl methacrylate – HE; 3-hydroxypropyl methacrylate – HP; and 2-trimethylammonium ethyl methacrylate chloride – T; and sulfobetaine methacrylate – S). The concentrations of the constituent monomers were 25, 30 or 35%. The water contact angles of the samples were measured, and half of the samples were exposed to saliva. The adherent yeast cells were counted after crystal violet staining.

Results

For the smooth samples, the groups S35, HP35 and HE35 showed significantly lower number of adhered Candida than control, in the absence of saliva. There were no significant differences among the experimental and control groups for the rough samples, but the saliva decreased the cell numbers for groups S25, S30 and HP30. The photoelectron spectroscopy analysis confirmed the changes in the chemical compositions of the experimental samples.

Conclusions

The experimental photopolymerised coatings changed the chemical composition and decreased C. albicans adhesion in the groups S35, HP35 and HE35, suggesting that they should be further investigated.

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