• 1
    Vignais PM, Billoud B & Meyer J (2001) Classification and phylogeny of hydrogenases. FEMS Microbiol Rev 25, 455501.
  • 2
    Vignais PM & Colbeau A (2004) Molecular biology of microbial hydrogenases. Curr Issues Mol Biol 6, 159188.
  • 3
    Adams MW (1990) The structure and mechanism of iron-hydrogenases. Biochim Biophys Acta 1020, 115145.
  • 4
    Albracht SPJ (2001) Spectroscopy-the functional puzzle. In Hydrogen as a Fuel. Learning from Nature (CammackR, FreyM & RobsonR, eds), pp. 110158. Taylor & Francis, London.
  • 5
    Cammack R (2001) The catalytic machinery. In Hydrogen as a Fuel. Learning from Nature (Cammack, R, FreyM & RobsonR, eds), pp. 159180. Taylor & Francis, London.
  • 6
    Happe RP, Roseboom W, Pierik AJ, Albracht SPJ & Bagley KA (1997) Biological activation of hydrogen. Nature 385, 126.
  • 7
    Bleijlevens B, Faber BW & Albracht SPJ (2001) The [Ni-Fe] hydrogenase from Allochromatium vinosum was studied in EPR-detectable states: H/D exchange experiments that yield new information about the structure of the active site. J Biol Inorg Chem 6, 763769.
  • 8
    Carepo M, Tierney DL, Brondino CD, Yang TC, Pamplona A, Teiser D, Moura I, Moura JJG & Hoffman BM (2002) 17O ENDOR detection of a solvent-derived Ni-(OHx) -Fe bridge that is lost upon activation of the hydrogenase from Desulfovibrio gigas. J Am Chem Soc 124, 281286.
  • 9
    Volbeda A, Garcin E, Piras C, De Lacey AL, Fernandez VM, Hatchikian EC, Frey M & Fontecilla-Camps JC (1996) Structure of the [NiFe]hydrogenase active site: evidence for uncommon Fe-ligands. J Am Chem Soc 118, 1298912996.
  • 10
    Garcin E, Vernede X, Hatchikian EC, Volbeda A, Frey M & Fontecilla-Camps JC (1999) The crystal structure of a reduced [NiFeSe]hydrogenase provides an image of the activated catalytic center. Structure Fold Des 7, 557566.
  • 11
    Higuchi Y, Ogata H, Miki K, Yasuoka N & Yagi T (1999) Removal of the bridging ligand atom at the Ni-Fe active site of [NiFe]hydrogenase upon reduction with H2, as revealed by X-ray structure analysis at 1.4 Å resolution. Structure Fold Des 7, 549556.
  • 12
    Elsen S, Colbeau A, Chabert J & Vignais PM (1996) The hupTUV operon is involved in negative control of hydrogenase synthesis in Rhodobacter capsulatus. J Bacteriol 178, 51745181.
  • 13
    Elsen S, Duché O & Colbeau A (2003) Interaction between the H2 sensor HupUV and the histidine kinase HupT controls HupSL hydrogenase synthesis in Rhodobacter capsulatus. J Bacteriol 185, 71117119.
  • 14
    Vignais PM, Dimon B, Zorin NA, Colbeau A & Elsen S (1997) HupUV proteins of Rhodobacter capsulatus can bind H2: evidence from the H-D exchange reaction. J Bacteriol 179, 290292.
  • 15
    Black LK, Fu C & Maier RJ (1994) Sequences and characterization of hupU and hupV genes of Bradyrhizobium japonicum encoding a possible nickel-sensing complex involved in hydrogenase expression. J Bacteriol 176, 71027106.
  • 16
    Bernhard M, Buhrke T, Bleijlevens B, De Lacey AJ, Fernandez VM, Albracht SPJ & Friedrich B (2001) The H2 sensor of Ralstonia eutropha. Biochemical characteristics, spectroscopic properties, and its interaction with a histidine protein kinase. J Biol Chem 276, 1559215597.
  • 17
    Kleihues L, Lenz O, Bernhard M, Buhrke T & Friedrich B (2000) The H2 sensor of Ralstonia eutropha is a member of the subclass of regulatory hydrogenases. J Bacteriol 182, 27162724.
  • 18
    Vignais PM, Cournac L, Hatchikian EC, Elsen S, Serebryakova N, Zorin NA & Dimon B (2002) Continuous monitoring of the activation and activity of [NiFe]-hydrogenases by membrane-inlet mass spectrometry. Int J Hydrogen Energy 27, 14411448.
  • 19
    Colbeau A & Vignais PM (1992) Use of hupS, lacZ gene fusion to study regulation of hydrogenase expression in Rhodobacter capsulatus: stimulation by H2. J Bacteriol 174, 42584264.
  • 20
    Dischert W, Vignais PM & Colbeau A (1999) The synthesis of Rhodobacter capsulatus HupSL hydrogenase is regulated by the two-component HupT/HupR system. Mol Microbiol 34, 9951006.
  • 21
    Buhrke T, Lenz O, Porthun A & Friedrich B (2004) The H2-sensing complex of Ralstonia eutropha: interaction between a regulatory [NiFe]hydrogenase and a histidine protein kinase. Mol Microbiol 51, 16771689.
  • 22
    Vignais PM, Dimon B, Zorin NA, Tomiyama M & Colbeau A (2000) Characterization of the hydrogen-deuterium exchange activities of the energy-transducing HupSL hydrogenase and H2-signaling HupUV hydrogenase in Rhodobacter capsulatus. J Bacteriol 182, 59976004.
  • 23
    Fontecilla-Camps JC, Frey M, Garcin E, Hatchikian C, Montet Y, Piras C, Vernede X & Volbeda A (1997) Hydrogenase: a hydrogen metabolizing enzyme. What do the crystal structures tell us about its mode of action. Biochimie 79, 661666.
  • 24
    Montet Y, Amara P, Volbeda A, Vernede X, Hatchikian EC, Field MJ, Frey M & Fontecilla-Camps JC (1997) Gas access to the active site of NiFe hydrogenases probed by X-ray crystallography and molecular dynamics. Nat Struct Biol 4, 523526.
  • 25
    Volbeda A, Montet Y, Vernede X, Hatchikian EC & Fontecilla-Camps JC (2002) High-resolution crystallographic analysis of Desulfovibrio fructosovorans[NiFe] hydrogenase. Int J Hydrogen Energy 27, 14491461.
  • 26
    Bryan JK (1977) Molecular weights of protein multimers from polyacrylamide gel electrophoresis. Anal Biochem 78, 513519.
  • 27
    Cammack R (2001) Hydrogenases and their activities. In Hydrogen as a Fuel. Learning from Nature (CammackR, FreyM & RobsonR, eds), pp. 7392. Taylor & Francis, London.
  • 28
    Hübner P, Willison JC, Vignais PM & Bickle TA (1991) Expression of regulatory nif genes in Rhodobacter capsulatus. J Bacteriol 173, 29932999.
  • 29
    Kurkin S, George SJ, Thorneley RNF & Albracht SPJ (2004) Hydrogen-induced activation of the [NiFe]-hydrogenase from Allochromatium vinosum as studied by stopped-flow infrared spectroscopy. Biochemistry 43, 68206831.
  • 30
    George SJ, Kurkin S, Thorneley RNF & Albracht SPJ (2004) Reaction of H2, CO, and O2 with active [NiFe]-hydrogenase from Allochromatium vinosum. A stopped-flow infrared study. Biochemistry 43, 68086819.
  • 31
    Schneider K, Schlegel HJ, Cammack R & Hall DO (1979) The iron-sulphur centres of soluble hydrogenase from Alcaligenes eutrophus. Biochim Biophys Acta 578, 445461.
  • 32
    Van der Linden E, Burgdorf T, Bernhard B, Bleijlevens B, Friedrich B & Albracht SPJ (2004) The soluble [NiFe]-hydrogenase from Ralstonia eutropha contains four cyanides in its active site, one of which is responsible for the insensitivity towards oxygen. J Biol Inorg Chem 9, 616626.
  • 33
    Happe RP, Roseboom W, Egert G, Friedrich CG, Massanz C, Friedrich B & Albracht SPJ (2000) Unusual FTIR and EPR properties of the H2-activating site of the cytoplasmic NAD-reducing hydrogenase from Ralstonia eutropha. FEBS Lett 466, 259263.
  • 34
    Buhrke T & Friedrich B (1998) hoxX (hypX) is a functional member of the Alcaligenes eutrophus hyp gene cluster. Arch Microbiol 170, 460463.
  • 35
    Bleijlevens B, Buhrke T, Van der Linden E, Friedrich B & Albracht SPJ (2004) The auxiliary protein HypX provides oxygen tolerance to the soluble [NiFe]-hydrogenase of Ralstonia eutropha H16 by way of a cyanide ligand to nickel. J Biol Chem 279, 4668646689.
  • 36
    Rey L, Fernandez D, Brito B, Hernando Y, Palacios JM, Imperial J & Ruiz-Argueso T (1996) The hydrogenase gene cluster of Rhizobium leguminosarum bv viciae contains an additional gene (hypX) which encodes a protein with sequence similarity to the N10-formyltetrahydrofolate-dependent enzyme family and is required for nickel-dependent hydrogenase processing and activity. Mol General Genet 252, 237248.
  • 37
    Burgdorf T, De Lacey AL & Friedrich B (2002) Functional analysis by site-directed mutagenesis of the NAD+-reducing hydrogenase from Ralstonia eutropha. J Bacteriol 184, 62806288.
  • 38
    Brecht M, Van Gastel M, Burhke T, Friedrich B & Lubitz W (2003) Direct detection of a hydrogen ligand in the [NiFe] center of the regulatory H2-sensing hydrogenase from Ralstonia eutropha in its reduced state by HYSCORE and ENDOR spectroscopy. J Am Chem Soc 125, 1307513083.
  • 39
    Haumann M, Porthun A, Buhrke T, Liebisch P, Meyer-Klaucke W, Friedrich B & Dau H (2003) Hydrogen-induced structural changes at the nickel site of the regulatory [NiFe]hydrogenase from Ralstonia eutropha detected by X-ray absorption spectroscopy. Biochemistry 42, 1100411015.
  • 40
    Buhrke T, Lenz O, Krauss N & Friedrich B (2005) Oxygen tolerance of the H2-sensing [NiFe] hydrogenase from Ralstoniae utropho H16 is based on limited access of oxygen to the active site. J Biol Chem in press.
  • 41
    Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring, N.Y.
  • 42
    Colbeau A, Godfroy A & Vignais PM (1986) Cloning of DNA fragments carrying hydrogenase genes of Rhodopseudomonas capsulata. Biochimie 68, 147155.
  • 43
    Colbeau A, Kelley BC & Vignais PM (1980) Hydrogenase activity in Rhodopseudomonas capsulata: relationship with nitrogenase activity. J Bacteriol 144, 141148.
  • 44
    Cournac L, Guedeney G, Peltier G & Vignais PM (2004) Sustained photoevolution of molecular hydrogen in a mutant of Synechocystis sp. strain PCC 6803 deficient in the type I NADPH-dehydrogenase complex. J Bacteriol 186, 17371746.
  • 45
    Marrs B (1974) Genetic recombination in Rhodopseudomonas capsulata. Proc Natl Acad Sci USA 71, 971973.
  • 46
    Colbeau A, Magnin JP, Cauvin B, Champion T & Vignais PM (1990) Genetic and physical mapping of an hydrogenase gene cluster from Rhodobacter capsulatus. Mol General Genet 220, 393399.
  • 47
    Yanisch-Perron C, Vieira J & Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103119.
  • 48
    Duport C, Meyer C, Naud I & Jouanneau Y (1994) A new gene expression system based on a fructose-dependent promoter from Rhodobacter capsulatus. Gene 145, 103108.
  • 49
    Ditta G, Stanfield S, Corbin D & Helinski DP (1980) Broad-host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77, 73477351.