• 1
    Zaehres H, Lensch MW, Daheron L, Stewart SA, Itskovitz-Eldor J & Daley GQ (2005) High-efficiency RNA interference in human embryonic stem cells. Stem Cells 23, 299305.
  • 2
    Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S & Smith A (2003) Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113, 643655.
  • 3
    Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M & Yamanaka S (2003) The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113, 631642.
  • 4
    Kuroda T, Tada M, Kubota H, Kimura H, Hatano SY, Suemori H, Nakatsuji N & Tada T (2005) Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression. Mol Cell Biol 25, 24752485.
  • 5
    Rodda DJ, Chew JL, Lim LH, Loh YH, Wang B, Ng HH & Robson P (2005) Transcriptional regulation of Nanog by OCT4 and SOX2. J Biol Chem 280, 2473124737.
  • 6
    Vanin EF (1985) Processed pseudogenes: characteristics and evolution. Annu Rev Genet 19, 253272.
  • 7
    Harrison PM, Hegyi H, Balasubramanian S, Luscombe NM, Bertone P, Echols N, Johnson T & Gerstein M (2002) Molecular fossils in the human genome: identification and analysis of the pseudogenes in chromosomes 21 and 22. Genome Res 12, 272280.
  • 8
    McCarrey JR & Riggs AD (1986) Determinator-inhibitor pairs as a mechanism for threshold setting in development: a possible function for pseudogenes. Proc Natl Acad Sci USA 83, 679683.
  • 9
    Korneev SA, Park JH & O'Shea M (1999) Neuronal expression of neural nitric oxide synthase (nNOS) protein is suppressed by an antisense RNA transcribed from an NOS pseudogene. J Neurosci 19, 77117720.
  • 10
    Hirotsune S, Yoshida N, Chen A, Garrett L, Sugiyama F, Takahashi S, Yagami K, Wynshaw-Boris A & Yoshiki A (2003) An expressed pseudogene regulates the messenger-RNA stability of its homologous coding gene. Nature 423, 9196.
  • 11
    Yano Y, Saito R, Yoshida N, Yoshiki A, Wynshaw-Boris A, Tomita M & Hirotsune S (2004) A new role for expressed pseudogenes as ncRNA: regulation of mRNA stability of its homologous coding gene. J Mol Med 82, 414422.
  • 12
    Booth HA & Holland PW (2004) Eleven daughters of NANOG. Genomics 84, 229238.
  • 13
    Pain D, Chirn GW, Strassel C & Kemp DM (2005) Multiple retropseudogenes from pluripotent cell-specific gene expression indicates a potential signature for novel gene identification. J Biol Chem 280, 62656268.
  • 14
    Takagi S, McFadden ML, Humphreys RE, Woda BA & Sairenji T (1993) Detection of 5-bromo-2-deoxyuridine (BrdUrd) incorporation with monoclonal anti-BrdUrd antibody after deoxyribonuclease treatment. Cytometry 14, 640648.
  • 15
    Mighell AJ, Smith NR, Robinson PA & Markham AF (2000) Vertebrate pseudogenes. FEBS Lett 468, 109114.
  • 16
    Ruud P, Fodstad O & Hovig E (1999) Identification of a novel cytokeratin 19 pseudogene that may interfere with reverse transcriptase-polymerase chain reaction assays used to detect micrometastatic tumor cells. Int J Cancer 80, 119125.
  • 17
    Normile D (2002) Cell proliferation. Common control for cancer, stem cells. Science 298, 1869.
  • 18
    Zhang JY, Wang X, Chen B, Suo GL, Zhao YH, Duan ZY & JW. Dai. (2005) Expression of Nanog gene promotes NIH3T3 cell proliferation. Biochem Biophys Res Commun 338, 10981102.
  • 19
    Monk M & Holding C (2001) Human embryonic genes re-expressed in cancer cells. Oncogene 20, 80858091.
  • 20
    Looijenga LH, Stoop H, de Leeuw HP, de Gouveia Brazao CA, Gillis AJ, van Roozendaal KE, van Zoelen EJ, Weber RF, Wolffenbuttel KP, van Dekken H et al. (2003) POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63, 22442250.
  • 21
    Tai MH, Chang CC, Kiupel M, Webster JD, Olson LK & Trosko JE (2005) Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis. Carcinogenesis 26, 495502.
  • 22
    Ezeh UI, Turek PJ, Reijo RA & Clark AT (2005) Human embryonic stem cell genes OCT4, NANOG, STELLAR, and GDF3 are expressed in both seminoma and breast carcinoma. Cancer 104, 22552265.
  • 23
    Hoei-Hansen CE, Almstrup K, Nielsen JE, Brask SS, Graem N, Skakkebaek NE, Leffers H & Rajpert-De Meyts E (2005) Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours. Histopathology 47, 4856.
  • 24
    Clark AT, Rodriguez RT, Bodnar MS, Abeyta MJ, Cedars MI, Turek PJ, Firpo MT & Reijo Pera RA (2005) Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma. Stem Cells 22, 169179.
  • 25
    Sadowski HB & Gilman MZ (1993) Cell-free activation of a DNA-binding protein by epidermal growth factor. Nature 362, 7983.
  • 26
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle protein-dye binding. Anal Biochem 72, 248254.