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Cell-free translation systems for protein engineering

  1. Yoshihiro Shimizu1,
  2. Yutetsu Kuruma2,
  3. Bei-Wen Ying1,
  4. So Umekage3,
  5. Takuya Ueda1

Article first published online: 23 AUG 2006

DOI: 10.1111/j.1742-4658.2006.05431.x

Issue

FEBS Journal

FEBS Journal

Volume 273, Issue 18, pages 4133–4140, September 2006

Additional Information

How to Cite

Shimizu, Y., Kuruma, Y., Ying, B.-W., Umekage, S. and Ueda, T. (2006), Cell-free translation systems for protein engineering. FEBS Journal, 273: 4133–4140. doi: 10.1111/j.1742-4658.2006.05431.x

Author Information

  1. 1

     Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa-shi, Chiba, Japan

  2. 2

     ‘Enrico Fermi’ Center, Compendio del Viminale, Rome, Italy

  3. 3

     Division of Bioscience and Biotechnology, Department of Ecological Engineering, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, Japan

*T. Ueda, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, FSB401, 5-1-5, Kashiwanoha, Kashiwa-shi, Chiba prefecture 277-8562, Japan Fax: +81 4 7136 3642 Tel: +81 4 7136 3641 E-mail: ueda@k.u-tokyo.ac.jp

Publication History

  1. Issue published online: 23 AUG 2006
  2. Article first published online: 23 AUG 2006
  3. (Received 8 May 2006, revised 20 June 2006, accepted 26 June 2006)

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Keywords:

  • cell-free protein synthesis;
  • chaperone;
  • disulfide bond formation;
  • in vitro selection;
  • liposome;
  • minimal cell;
  • ribosome display;
  • translation;
  • unnatural amino acid

Cell-free translation systems have developed significantly over the last two decades and improvements in yield have resulted in their use for protein production in the laboratory. These systems have protein engineering applications, such as the production of proteins containing unnatural amino acids and development of proteins exhibiting novel functions. Recently, it has been suggested that cell-free translation systems might be used as the fundamental basis for cell-like systems. We review recent progress in the field of cell-free translation systems and describe their use as tools for protein production and engineering.

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