Cell-free translation systems for protein engineering


T. Ueda, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, FSB401, 5-1-5, Kashiwanoha, Kashiwa-shi, Chiba prefecture 277-8562, Japan Fax: +81 4 7136 3642 Tel: +81 4 7136 3641 E-mail: ueda@k.u-tokyo.ac.jp


Cell-free translation systems have developed significantly over the last two decades and improvements in yield have resulted in their use for protein production in the laboratory. These systems have protein engineering applications, such as the production of proteins containing unnatural amino acids and development of proteins exhibiting novel functions. Recently, it has been suggested that cell-free translation systems might be used as the fundamental basis for cell-like systems. We review recent progress in the field of cell-free translation systems and describe their use as tools for protein production and engineering.