• 1
    Banerjee RV, Frasca V, Ballou DP & Matthews RG (1990) Participation of cob(I)alamin in the reaction catalyzed by methionine synthase from Escherichia coli: a steady-state and rapid reaction kinetic analysis. Biochemistry 29, 1110111109.
  • 2
    Fujii K, Galivan JH & Huennekens FM (1977) Activation of methionine synthase: further characterization of flavoprotein system. Arch Biochem Biophys 178, 662670.
  • 3
    Olteanu H & Banerjee R (2001) Human methionine synthase reductase, a soluble P-450 reductase-like dual flavoprotein, is sufficient for NADPH-dependent methionine synthase activation. J Biol Chem 276, 3555835563.
  • 4
    Peariso K, Zhou ZS, Smith AE, Matthews RG & Penner-Hahn JE (2001) Characterization of the zinc sites in cobalamin-independent and cobalamin-dependent methionine synthase using zinc and selenium X-ray absorption spectroscopy. Biochemistry 40, 987993.
  • 5
    Evans JC, Huddler DP, Hilgers MT, Romanchuk G, Matthews RG & Ludwig ML (2004) Structures of the N-terminal modules imply large domain motions during catalysis by methionine synthase. Proc Natl Acad Sci USA 101, 37293736.
  • 6
    Wolthers KR, Toogood HS, Jowitt TA, Marshall KR, Leys D & Scrutton NS (2007) Crystal structure and solution characterization of the activation domain of human methionine synthase. FEBS J 274, 738750.
  • 7
    Drennan CL, Huang S, Drummond JT, Matthews RG & Lidwig ML (1994) How a protein binds B12: a 3.0 A X-ray structure of B12-binding domains of methionine synthase. Science 266, 16691674.
  • 8
    Dixon MM, Huang S, Matthews RG & Ludwig M (1996) The structure of the C-terminal domain of methionine synthase: presenting S-adenosylmethionine for reductive methylation of B12. Structure 4, 12631275.
  • 9
    Leclerc D, Odievre M, Wu Q, Wilson A, Huizenga JJ, Rozen R, Scherer SW & Gravel RA (1999) Molecular cloning, expression and physical mapping of the human methionine synthase reductase gene. Gene 240, 7588.
  • 10
    Wang M, Roberts DL, Paschke R, Shea TM, Masters BS & Kim JJ (1997) Three-dimensional structure of NADPH-cytochrome P450 reductase: prototype for FMN- and FAD-containing enzymes. Proc Natl Acad Sci USA 94, 84118416.
  • 11
    Garcin ED, Bruns CM, Lloyd SJ, Hosfield DJ, Tiso M, Gachhui R, Stuehr DJ, Tainer JA & Getzoff ED (2004) Structural basis for isozyme-specific regulation of electron transfer in nitric-oxide synthase. J Biol Chem 279, 3791837927.
  • 12
    Bredt DS, Hwang PM, Glatt CE, Lowenstein C, Reed RR & Snyder SH (1991) Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. Nature 351, 714718.
  • 13
    Paine MJ, Garner AP, Powell D, Sibbald J, Sales M, Pratt N, Smith T, Tew DG & Wolf CR (2000) Cloning and characterization of a novel human dual flavin reductase. J Biol Chem 275, 14711478.
  • 14
    Yamada K, Gravel RA, Toraya T & Matthews RG (2006) Human methionine synthase reductase is a molecular chaperone for human methionine synthase. Proc Natl Acad Sci USA 103, 94769481.
  • 15
    Olteanu H & Banerjee R (2003) Redundancy in the pathway for redox regulation of mammalian methionine synthase: reductive activation by the dual flavoprotein, novel reductase 1. J Biol Chem 278, 3831038314.
  • 16
    Suliman HS, Sawyer GM, Appling DR & Robertus JD (2005) Purification and properties of cobalamin-independent methionine synthase from Candida albicans and Saccharomyces cerevisiae. Arch Biochem Biophys 441, 5663.
  • 17
    Wolthers KR, Lou X, Toogood HS, Leys D & Scrutton NS (2007) Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry. Biochemistry 46, 1183311844.
  • 18
    Wolthers KR & Scrutton NS (2004) Electron transfer in human methionine synthase reductase studied by stopped-flow spectrophotometry. Biochemistry 43, 490500.
  • 19
    Wolthers KR & Scrutton NS (2007) Protein interactions in the human methionine synthase–methionine synthase reductase complex and implications for the mechanism of enzyme reactivation. Biochemistry 46, 66966709.
  • 20
    Jarrett JT, Choi CY & Matthews RG (1997) Changes in protonation associated with substrate binding and cob(I)alamin formation in cobalamin-dependent methionine synthase. Biochemistry 36, 1573915748.
  • 21
    Wolthers KR, Basran J, Munro AW & Scrutton NS (2003) Molecular dissection of human methionine synthase reductase: determination of the flavin redox potentials in full-length enzyme and isolated flavin-binding domains. Biochemistry 42, 39113920.
  • 22
    Hoover DM, Jarrett JT, Sands RH, Dunham WR, Ludwig ML & Matthews RG (1997) Interaction of Escherichia coli cobalamin-dependent methionine synthase and its physiological partner flavodoxin: binding of flavodoxin leads to axial ligand dissociation from the cobalamin cofactor. Biochemistry 36, 127138.
  • 23
    Banerjee RV, Harder SR, Ragsdale SW & Matthews RG (1990) Mechanism of reductive activation of cobalamin-dependent methionine synthase: an electron paramagnetic resonance spectroelectrochemical study. Biochemistry 29, 11291135.
  • 24
    Chen Z, Chakraborty S & Banerjee R (1995) Demonstration that mammalian methionine synthases are predominantly cobalamin-loaded. J Biol Chem 270, 1924619249.
  • 25
    Yamada K, Yamada S, Tobimatsu T & Toraya T (1999) Heterologous high level expression, purification, and enzymological properties of recombinant rat cobalamin-dependent methionine synthase. J Biol Chem 274, 3557135576.
  • 26
    Leal NA, Olteanu H, Banerjee R & Bobik TA (2004) Human ATP:cob(I)alamin adenosyltransferase and its interaction with methionine synthase reductase. J Biol Chem 279, 4753647542.
  • 27
    Sato K, Hiei E & Shimizu S (1978) Affinity chromatography of N5-methyltetrahydrofolate-homocysteine methyltransferase on a cobalamin-Sepharose. FEBS Lett 85, 7376.
  • 28
    Modi S, Gilham DE, Sutcliffe MJ, Lian LY, Primrose WU, Wolf CR & Roberts GC (1997) 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine as a substrate of cytochrome P450 2D6: allosteric effects of NADPH-cytochrome P450 reductase. Biochemistry 36, 44614470.
  • 29
    Knight K & Scrutton NS (2002) Stopped-flow kinetic studies of electron transfer in the reductase domain of neuronal nitric oxide synthase: re-evaluation of the kinetic mechanism reveals new enzyme intermediates and variation with cytochrome P450 reductase. Biochem J 367, 1930.
  • 30
    Jarrett JT, Goulding CW, Fluhr K, Huang S & Matthews RG (1997) Purification and assay of cobalamin-dependent methionine synthase from Escherichia coli. Meth Enzymol 281, 196213.
  • 31
    Watanabe F & Nakano Y (1997) Purification and characterization of aquacobalamin reductase from Euglena gracilis. Meth Enzymol 281, 289295.
  • 32
    Gutierrez A, Lian LY, Wolf CR, Scrutton NS & Roberts GC (2001) Stopped-flow kinetic studies of flavin reduction in human cytochrome P450 reductase and its component domains. Biochemistry 40, 19641975.
  • 33
    Bandarian V, Pattridge KA, Lennon BW, Huddler DP, Matthews RG & Ludwig ML (2002) Domain alternation switches B(12)-dependent methionine synthase to the activation conformation. Nat Struct Biol 9, 5356.
  • 34
    Tollin G (1995) Use of flavin photochemistry to probe intraprotein and interprotein electron transfer mechanisms. J Bioenerg Biomembr 27, 303309.