Anaerobic sulfatase-maturating enzyme – A mechanistic link with glycyl radical-activating enzymes?
Article first published online: 9 MAR 2010
© 2010 The Authors Journal compilation © 2010 FEBS
Volume 277, Issue 8, pages 1906–1920, April 2010
How to Cite
Benjdia, A., Subramanian, S., Leprince, J., Vaudry, H., Johnson, M. K. and Berteau, O. (2010), Anaerobic sulfatase-maturating enzyme – A mechanistic link with glycyl radical-activating enzymes?. FEBS Journal, 277: 1906–1920. doi: 10.1111/j.1742-4658.2010.07613.x
- Issue published online: 30 MAR 2010
- Article first published online: 9 MAR 2010
- (Received 8 October 2009, revised 22 December 2009, accepted 8 February 2010)
Fig. S1. MALDI-TOF MS analysis of 17 amino acid peptides after incubation with anSMEcpe.
Fig. S2. MALDI-TOF MS analysis of 17C and 17S peptides after incubation with anSMEcpe.
Fig. S3. Gel electrophoresis analysis (SDS PAGE 12.5%) of WT and the M1, M2 and M3 variants of anSMEbt.
Fig. S4. UV-visible absorption spectra of oxidized and dithionite-reduced as purified and reconstituted forms of WT and M1 mutant anSMEbt.
Fig. S5. Low field X-band EPR spectra of dithionite-reduced WT anSMEcpe in the presence or absence of a 20-fold excess of AdoMet.
Fig. S6. MALDI-TOF MS analysis of 17C peptide after incubation with wild type (WT) or M1, M2 and M3 mutants of anSMEbt.
Fig. S7. MALDI-TOF mass spectrometry analysis of 17C peptide before and after a 4 hours incubation with anSMEcpe in H216O (2) or H218O (3) buffer.
Please note: As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
|FEBS_7613_sm_Figures_S1-S7.pdf||334K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.