Advertisement

CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site

Authors

  • Karen Duus,

    1.  Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Artillerivej 5, Copenhagen, Denmark
    Search for more papers by this author
  • Nicole M. Thielens,

    1.  Laboratoire d’Enzymologie Moléculaire, Institut de Biologie Structurale Jean-Pierre Ebel, Commissariat à l’Energie Atomique, CNRS UMR 5075, Université Joseph Fourier, 41 rue Jules Horowitz, Grenoble Cedex 1, France
    Search for more papers by this author
  • Monique Lacroix,

    1.  Laboratoire d’Enzymologie Moléculaire, Institut de Biologie Structurale Jean-Pierre Ebel, Commissariat à l’Energie Atomique, CNRS UMR 5075, Université Joseph Fourier, 41 rue Jules Horowitz, Grenoble Cedex 1, France
    Search for more papers by this author
  • Pascale Tacnet,

    1.  Laboratoire d’Enzymologie Moléculaire, Institut de Biologie Structurale Jean-Pierre Ebel, Commissariat à l’Energie Atomique, CNRS UMR 5075, Université Joseph Fourier, 41 rue Jules Horowitz, Grenoble Cedex 1, France
    Search for more papers by this author
  • Philippe Frachet,

    1.  Laboratoire d’Enzymologie Moléculaire, Institut de Biologie Structurale Jean-Pierre Ebel, Commissariat à l’Energie Atomique, CNRS UMR 5075, Université Joseph Fourier, 41 rue Jules Horowitz, Grenoble Cedex 1, France
    Search for more papers by this author
  • Uffe Holmskov,

    1.  Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark
    Search for more papers by this author
  • Gunnar Houen

    1.  Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Artillerivej 5, Copenhagen, Denmark
    Search for more papers by this author

K. Duus, Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen, Denmark
Fax: +45 32683149
Tel: +45 32688241
E-mail: kds@ssi.dk

Abstract

CD91 plays an important role in the scavenging of apoptotic material, possibly through binding to soluble pattern-recognition molecules. In this study, we investigated the interaction of CD91 with mannan-binding lectin (MBL), ficolins and lung surfactant proteins. Both MBL and L-ficolin were found to bind CD91. The MBL–CD91 interaction was time- and concentration-dependent and could be inhibited by known ligands of CD91. MBL-associated serine protease 3 (MASP-3) also inhibited binding between MBL and CD91, suggesting that the site of interaction is located at or near the MASP–MBL interaction site. This was confirmed by using MBL mutants deficient for MASP binding that were unable to interact with CD91. These findings demonstrate that MBL and L-ficolin interact with CD91, strongly suggesting that they have the potential to function as soluble recognition molecules for scavenging microbial and apoptotic material by CD91.

Structured digital abstract

Ancillary