Broad antibiotic resistance profile of the subclass B3 metallo-β-lactamase GOB-1, a di-zinc enzyme
Article first published online: 4 MAR 2011
© 2011 The Authors Journal compilation © 2011 FEBS
Volume 278, Issue 8, pages 1252–1263, April 2011
How to Cite
Horsfall, L. E., Izougarhane, Y., Lassaux, P., Selevsek, N., Liénard, B. M. R., Poirel, L., Kupper, M. B., Hoffmann, K. M., Frère, J.-M., Galleni, M. and Bebrone, C. (2011), Broad antibiotic resistance profile of the subclass B3 metallo-β-lactamase GOB-1, a di-zinc enzyme. FEBS Journal, 278: 1252–1263. doi: 10.1111/j.1742-4658.2011.08046.x
- Issue published online: 1 APR 2011
- Article first published online: 4 MAR 2011
- Accepted manuscript online: 7 FEB 2011 10:09AM EST
- (Received 16 September 2010, revised 5 January 2011, accepted 4 February 2011)
Fig. S1. GOB-1 sequence, including the LNA from the signal peptide. Peptides from the tryptic digestion identified by MALDI-TOF MS are highlighted to show the sequence coverage. Peptides either have one or no sites of missed cleavage; no peptides with two or more missed cleavage sites were identified.
Fig. S2. Relative activity of the Q116N mutant measured in the presence of increasing concentrations of zinc.
Fig. S3. Native ESI-TOF MS of the remetallated GOB-1.
Fig. S4. Graph showing the effect of varying EDTA concentration on the ki of the GOB-1 enzymes.
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