Fig. S1. GOB-1 sequence, including the LNA from the signal peptide. Peptides from the tryptic digestion identified by MALDI-TOF MS are highlighted to show the sequence coverage. Peptides either have one or no sites of missed cleavage; no peptides with two or more missed cleavage sites were identified.

Fig. S2. Relative activity of the Q116N mutant measured in the presence of increasing concentrations of zinc.

Fig. S3. Native ESI-TOF MS of the remetallated GOB-1.

Fig. S4. Graph showing the effect of varying EDTA concentration on the ki of the GOB-1 enzymes.

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