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Fig. S1. Structural comparison of polyol substrates of AfM2DH.

Fig. S2. Comparison of the structural model of AfM2DH with the experimentally determined crystal structure of PsM2DH (1lj8).

Fig. S3. Kinetic analysis of inhibition of AfM1PDH (A–F) and AfM2DH (G–L) by adenine nucleotides at pH 7.1.

Fig. S4. Michaelis–Menten plot for Fru reduction by AfM2DH employing NADH or NADD.

Fig. S5. Irreversible inactivation of AfM1PDH (A, B) and AfM2DH (C, D) at different temperatures, represented as semilogarithmic plots.

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