Proteomic tools for the analysis of transient interactions between metalloproteins

Authors

  • Jonathan Martínez-Fábregas,

    1. Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Centro de Investigaciones Científicas Isla de la Cartuja, Sevilla, Spain
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  • Silvia Rubio,

    1. Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Centro de Investigaciones Científicas Isla de la Cartuja, Sevilla, Spain
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  • Antonio Díaz-Quintana,

    1. Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Centro de Investigaciones Científicas Isla de la Cartuja, Sevilla, Spain
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  • Irene Díaz-Moreno,

    1. Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Centro de Investigaciones Científicas Isla de la Cartuja, Sevilla, Spain
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  • Miguel Á. De la Rosa

    1. Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Centro de Investigaciones Científicas Isla de la Cartuja, Sevilla, Spain
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I. Díaz-Moreno, Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Centro de Investigaciones Científicas Isla de la Cartuja, Avenida Américo Vespucio 49, Sevilla 41092, Spain
Fax: +34 954460065
Tel: +34 954489513
E-mail: idiazmoreno@us.es

Abstract

Metalloproteins play major roles in cell metabolism and signalling pathways. In many cases, they show moonlighting behaviour, acting in different processes, depending on the physiological state of the cell. To understand these multitasking proteins, we need to discover the partners with which they carry out such novel functions. Although many technological and methodological tools have recently been reported for the detection of protein interactions, specific approaches to studying the interactions involving metalloproteins are not yet well developed. The task is even more challenging for metalloproteins, because they often form short-lived complexes that are difficult to detect. In this review, we gather the different proteomic techniques and biointeractomic tools reported in the literature. All of them have shown their applicability to the study of transient and weak protein–protein interactions, and are therefore suitable for metalloprotein interactions.

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