Proteomic reactors and their applications in biology

Authors

  • Hu Zhou,

    1.  Ottawa Institute of Systems Biology (OISB), University of Ottawa, ON, Canada
    2.  Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Canada
    3.  Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China
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  • Zhibin Ning,

    1.  Ottawa Institute of Systems Biology (OISB), University of Ottawa, ON, Canada
    2.  Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Canada
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  • Fangjun Wang,

    1.  Ottawa Institute of Systems Biology (OISB), University of Ottawa, ON, Canada
    2.  Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Canada
    3.  Key Lab of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, China
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  • Deeptee Seebun,

    1.  Ottawa Institute of Systems Biology (OISB), University of Ottawa, ON, Canada
    2.  Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Canada
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  • Daniel Figeys

    1.  Ottawa Institute of Systems Biology (OISB), University of Ottawa, ON, Canada
    2.  Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Canada
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  • Note
    Hu Zhou and Zhibin Ning contributed equally to this review

D. Figeys, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 451 Smyth Road, Ottawa, ON, Canada K1H 8M5
Fax: +1 613 562 5655
Tel: +1 613 562 5800 ext 8674
E-mail: dfigeys@uottawa.ca

Abstract

Proteomic analysis requires the combination of an extensive suite of technologies including protein processing and separation, micro-flow HPLC, MS and bioinformatics. Although proteomic technologies are still in flux, approaches that bypass gel electrophoresis (gel-free approaches) are dominating the field of proteomics. Along with the development of gel-free proteomics, came the development of devices for the processing of proteomic samples termed proteomic reactors. These microfluidic devices provide rapid, robust and efficient pre-MS sample procession by performing protein sample preparation/concentration, digestion and peptide fractionation. The proteomic reactor has advanced in two major directions: immobilized enzyme reactor and ion exchange-based proteomic reactor. This review summarizes the technical developments and biological applications of the proteomic reactor over the last decade.

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