Fig. S1. Quantitative real-time PCR determination of hoxE, hoxF and hoxH expression in the four strains used during this study.

Fig. S2. Detection of hydrogenase activity by colorimetric (MV reduction) assay after electrophoresis in nondenaturing polyacrylamide gels.

Fig. S3. Effects of ferredoxin and ferredoxin–NADP+ reductase (FNR) addition on NADPH-mediated hydrogenase activation in Synechocystis extracts.

Table S1. List of primers used in this study.

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