A novel method for monitoring the localization of cytochromes P450 and other endoplasmic reticulum membrane associated proteins: a tool for investigating the formation of metabolons
Version of Record online: 15 SEP 2011
© 2011 The Authors Journal compilation © 2011 FEBS
The FEBS Journal
Special Issue: Cytochrome P450 Structure and Function
Volume 279, Issue 9, pages 1576–1583, May 2012
How to Cite
Bassard, J.-E., Mutterer, J., Duval, F. and Werck-Reichhart, D. (2012), A novel method for monitoring the localization of cytochromes P450 and other endoplasmic reticulum membrane associated proteins: a tool for investigating the formation of metabolons. The FEBS Journal, 279: 1576–1583. doi: 10.1111/j.1742-4658.2011.08312.x
- Issue online: 19 APR 2012
- Version of Record online: 15 SEP 2011
- Accepted manuscript online: 18 AUG 2011 02:48PM EST
- (Received 24 June 2011, revised 9 August 2011, accepted 16 August 2011)
Table S1. Text file of the macro for imagej.
Table S2. Reliability of results from Figs 1 and 7.
Table S3. Limits of the technique related to inclination of the measure line.
Table S4. Limits of the method due to over-exposure of the fluoresence detector and to change in the optical slice.
Table S5. Details of primers for pCAMBIA230035SueGFP and pCAMBIA330035SumRFP1 construction.
Table S6. List of primers for USER™ cloning.
Table S7. DNA and protein sequence of the CYP51G1 anchor-eGFP fusion (referred as ‘anchored eGFP’).
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