Fig. S1. Nucleotide sequence of the nikK gene and amino acid sequence of NikK.

Fig. S2. Alignment of the amino acid sequences of NikK from Streptomyces tendae, histidinol phosphate cuminotransferase (HisC) from Escherichia coli, aspartate aminotransferase (AAT) from E. coli and SanB from S. ansochromogenes.

Fig. S3. Gel filtration chromatogram of NikK.

Fig. S4. SDS gel of NikK.

Doc. S1. Crystallization trials with wild-type NikK as well as a surface entropy reduction mutant version of the enzyme.

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