Characterization of the PLP-dependent aminotransferase NikK from Streptomyces tendae and its putative role in nikkomycin biosynthesis
Article first published online: 28 SEP 2011
© 2011 The Authors Journal compilation © 2011 FEBS
Volume 278, Issue 21, pages 4122–4135, November 2011
How to Cite
Binter, A., Oberdorfer, G., Hofzumahaus, S., Nerstheimer, S., Altenbacher, G., Gruber, K. and Macheroux, P. (2011), Characterization of the PLP-dependent aminotransferase NikK from Streptomyces tendae and its putative role in nikkomycin biosynthesis. FEBS Journal, 278: 4122–4135. doi: 10.1111/j.1742-4658.2011.08319.x
- Issue published online: 21 OCT 2011
- Article first published online: 28 SEP 2011
- Accepted manuscript online: 2 SEP 2011 12:00AM EST
- (Received 6 July 2011, revised 29 August 2011, accepted 30 August 2011)
Fig. S1. Nucleotide sequence of the nikK gene and amino acid sequence of NikK.
Fig. S2. Alignment of the amino acid sequences of NikK from Streptomyces tendae, histidinol phosphate cuminotransferase (HisC) from Escherichia coli, aspartate aminotransferase (AAT) from E. coli and SanB from S. ansochromogenes.
Fig. S3. Gel filtration chromatogram of NikK.
Fig. S4. SDS gel of NikK.
Doc. S1. Crystallization trials with wild-type NikK as well as a surface entropy reduction mutant version of the enzyme.
Please note: As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
|FEBS_8319_sm_FigS1-S4-DocS1.zip||1082K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.