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Fig. S1. Absorbance and CD spectra of PpoA_wt and various mutants.

Fig. S2. SDS/PAGE of purified PpoA wild type and variants.

Fig. S3. Identification of the peak eluting at 16.7 min as methyl ester of 5,8-DiHODE.

Fig. S4. 9-GHz EPR spectra of PpoA (75 μm) and N887V (75 μm).

Fig. S5. (A) 9-GHz CW spectrum at 70 K of the radical formed after reaction of either PpoA (75 μm) (black) or Y327F (blue) with 160-fold excess (8R)-HPODE. (B) 95-GHz Echo-detected spectrum (1-st derivative) of the radical formed after reaction of either PpoA (75 mm) (black) or Y327F (blue) with 160-fold excess (8R)-HPODE.

Fig. S6. Superimposition of the modeled DOX domain of PpoA and oPGHS-1.

Fig. S7. Superimposition of the structures of Arabidopsis thalania and Parthenium argentatum AOS.

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