Present addresses Division of Molecular Plant Physiology and Biophysics, Botany I, Julius-von-Sachs-Institute of Biological Sciences, Julius-Maximilian-University Würzburg, Würzburg, Germany
Catalytically active filaments – pyruvate decarboxylase from Neurospora crassa. pH-controlled oligomer structure and catalytic function
Article first published online: 9 DEC 2011
Journal compilation © 2011 FEBS. No claim to original German government works
Volume 279, Issue 2, pages 275–284, January 2012
How to Cite
Hüttl, S., Fiebig, J., Kutter, S., Hause, G., Lilie, H., Spinka, M. and König, S. (2012), Catalytically active filaments – pyruvate decarboxylase from Neurospora crassa. pH-controlled oligomer structure and catalytic function. FEBS Journal, 279: 275–284. doi: 10.1111/j.1742-4658.2011.08421.x
Note Stefanie Hüttl and Juliane Fiebig contributed equally to this work
- Issue published online: 3 JAN 2012
- Article first published online: 9 DEC 2011
- Accepted manuscript online: 12 NOV 2011 11:08AM EST
- (Received 17 June 2011, revised 3 November 2011, accepted 10 November 2011)
Fig. S1 SDS/PAGE of NcPDC.
Fig. S2 Selected progress curves of the NcPDC catalysed reaction at pH 5.0–7.0 (from left to right, top to bottom, pH 5.0, 5.5, 6.0, 6.5, 7.0).
Fig. S3.v/[S] plots of the NcPDC catalysed reaction at pH 5.5 (left) and 6.5 (right).
Fig. S4. Analytical ultracentrifugation runs of NcPDC solution at 0.3 mg·mL−1 20 °C at pH 6.5–7.5.
Fig. S5. Electron micrographs of PsPDC at pH 6.5.
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