Cloning, expression and characterization of CYP102D1, a self-sufficient P450 monooxygenase from Streptomyces avermitilis
Article first published online: 23 JAN 2012
© 2011 The Authors Journal compilation © 2011 FEBS
Special Issue: Cytochrome P450 Structure and Function
Volume 279, Issue 9, pages 1650–1662, May 2012
How to Cite
Choi, K.-Y., Jung, E., Jung, D.-H., Pandey, B. P., Yun, H., Park, H.-y., Kazlauskas, R. J. and Kim, B.-G. (2012), Cloning, expression and characterization of CYP102D1, a self-sufficient P450 monooxygenase from Streptomyces avermitilis. FEBS Journal, 279: 1650–1662. doi: 10.1111/j.1742-4658.2011.08462.x
- Issue published online: 19 APR 2012
- Article first published online: 23 JAN 2012
- Accepted manuscript online: 20 DEC 2011 01:06PM EST
- (Received 25 August 2011, revised 17 November 2011, accepted 12 December 2011)
Fig. S1. DNA sequences synthesized based on the E. coli codon bias for heterologous expression.
Fig. S2. Extraction and separation of diflavins from CYP102D1 and catalytic activity of diflavin reductase.
Fig. S3. Substrate binding to CYP102D1 and hyperbolic fitting of substrate-induced heme spectral changes.
Fig. S4. Separation of indole reaction products by GC/MS catalysed by CYP102D1 to produce 5-hydroxyindole or 6-hydroxyindole.
Fig. S5. HPLC analysis of oxidative metabolites of daidzein by CYP102D1 mutants.
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