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Fig. S1. DNA sequences synthesized based on the E. coli codon bias for heterologous expression.

Fig. S2. Extraction and separation of diflavins from CYP102D1 and catalytic activity of diflavin reductase.

Fig. S3. Substrate binding to CYP102D1 and hyperbolic fitting of substrate-induced heme spectral changes.

Fig. S4. Separation of indole reaction products by GC/MS catalysed by CYP102D1 to produce 5-hydroxyindole or 6-hydroxyindole.

Fig. S5. HPLC analysis of oxidative metabolites of daidzein by CYP102D1 mutants.

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