Fig. S1. (A), (B) Time course assay ofPII-P dephosphorylation by tPphA variants. (C) Timecourse assay of PII-P dephosphorylation by tPphA, humanPP2Cα and chimera A.

Fig. S2. Mn2+binding to tPphA,chimera A and human PP2Cα studied by isothermal titration calorimetry.

Fig. S3. (A) Inhibitory effect ofCa2+ on the relative activities of tPphA towardspNPP, pT-peptide, T-loop peptide and PII-P. (B)Inhibitory effect of Ca2+ on the dephosphorylation ofPII-P by wild-type tPphA.

Fig. S4. Glutaraldehyde (GDA) crosslinkingfollowed by Ni-NTA affinity purification (pull-down, PD) usingHis-tPphA (10 μg) with PII-P(0.5 μg).

Fig. S5. Recovery of tPphA from the pull-down assays shown in Fig. 6(A).

Table S1. The affinity and thermodynamicparameters of tPphA, chimera A and human PP2Cα from ITC assay.

Table S2. Primers used for PCR amplification of tPphA and for site-directed mutagenesis.

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