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Fig. S1. Analyses of the interaction between p60-vMIT and p80-CTD.

Fig. S2. ATPase activity of full-length p60-katanin in the presence of MT, p80-CTD, and increasing concentrations of Ca2+.

Fig. S3.1H-15N HSQC spectra of p60-vMIT in the absence (black) and presence of 5 mM (magenta), 10 mM (cyan), 15 mM (yellow), 25 mM (green) Ca2+.

Fig. S4. Ce3+ titration experiments of p60-vMIT.

Fig. S5. Close-up view of the Ca2+ binding region of p60-katanin in Fig. 6C.

Fig. S6. MT co-sedimentation assay in the presence of p60-katanin and p80-CTD in vitro.

Table S1. The broadened signals of 15N-labeled p60-vMIT in the presence of p80-CTD.

Table S2. Oligonucleotides used as primers for Ala substitution.

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