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Fig. S1. The binding of zinc.

Fig. S2. XRF-spectra of Bc-β-gal. The energy is in keV.

Fig. S3. Energy scan around the absorption edge of Zn. The energy is in keV.

Fig. S4. Dynamic light scattering results of Bc-β-gal.

Fig. S5. The packing of C4 of galactose against F345.

Fig. S6. The observed conformational changes between the free (in grey) and galactose-bound (in green) structures of Bc-β-gal.

Fig. S7. Crystals of Bc-β-gal. (A) twinned crystals (B) stick crystals.

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