Structural analysis, enzymatic characterization, and catalytic mechanisms of β-galactosidase from Bacillus circulans sp. alkalophilus
Version of Record online: 28 MAR 2012
© 2012 The Authors Journal compilation © 2012 FEBS
The FEBS Journal
Volume 279, Issue 10, pages 1788–1798, May 2012
How to Cite
Maksimainen, M., Paavilainen, S., Hakulinen, N. and Rouvinen, J. (2012), Structural analysis, enzymatic characterization, and catalytic mechanisms of β-galactosidase from Bacillus circulans sp. alkalophilus. The FEBS Journal, 279: 1788–1798. doi: 10.1111/j.1742-4658.2012.08555.x
- Issue online: 23 APR 2012
- Version of Record online: 28 MAR 2012
- Accepted manuscript online: 3 MAR 2012 07:53AM EST
- (Received 3 January 2012, revised 23 February 2012, accepted 28 February 2012)
Fig. S1. The binding of zinc.
Fig. S2. XRF-spectra of Bc-β-gal. The energy is in keV.
Fig. S3. Energy scan around the absorption edge of Zn. The energy is in keV.
Fig. S4. Dynamic light scattering results of Bc-β-gal.
Fig. S5. The packing of C4 of galactose against F345.
Fig. S6. The observed conformational changes between the free (in grey) and galactose-bound (in green) structures of Bc-β-gal.
Fig. S7. Crystals of Bc-β-gal. (A) twinned crystals (B) stick crystals.
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