Sustained glycolytic oscillations in individual isolated yeast cells
Article first published online: 11 JUN 2012
© 2012 The Authors Journal compilation © 2012 FEBS
Volume 279, Issue 16, pages 2837–2847, August 2012
How to Cite
Gustavsson, A.-K., van Niekerk, D. D., Adiels, C. B., du Preez, F. B., Goksör, M. and Snoep, J. L. (2012), Sustained glycolytic oscillations in individual isolated yeast cells. FEBS Journal, 279: 2837–2847. doi: 10.1111/j.1742-4658.2012.08639.x
- Issue published online: 24 JUL 2012
- Article first published online: 11 JUN 2012
- Accepted manuscript online: 18 MAY 2012 11:00AM EST
- (Received 19 January 2012, revised 3 April 2012, accepted 11 May 2012)
- cell heterogeneity;
- limit-cycle oscillation;
- mathematical model;
- microfluidic chamber
Yeast glycolytic oscillations have been studied since the 1950s in cell-free extracts and intact cells. For intact cells, sustained oscillations have so far only been observed at the population level, i.e. for synchronized cultures at high biomass concentrations. Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simulated the heterogeneity in the response of the individual cells, assuming small differences in a single internal parameter. This is the first time that sustained limit-cycle oscillations have been demonstrated in isolated yeast cells.
Database The mathematical model described here has been submitted to the JWS Online Cellular Systems Modelling Database and can be accessed at http://jjj.biochem.sun.ac.za/database/gustavsson/index.html free of charge.