Production and characterization of scFvA33T1, an immunoRNase targeting colon cancer cells
Article first published online: 25 JUL 2012
© 2012 The Authors Journal compilation © 2012 FEBS
Volume 279, Issue 17, pages 3022–3032, September 2012
How to Cite
Tomé-Amat, J., Menéndez-Méndez, A., García-Ortega, L., Batt, C. A., Oñaderra, M., Martínez-del-Pozo, Á., Gavilanes, J. G. and Lacadena, J. (2012), Production and characterization of scFvA33T1, an immunoRNase targeting colon cancer cells. FEBS Journal, 279: 3022–3032. doi: 10.1111/j.1742-4658.2012.08683.x
- Issue published online: 21 AUG 2012
- Article first published online: 25 JUL 2012
- Accepted manuscript online: 2 JUL 2012 03:35PM EST
- (Received 13 January 2012, revised 14 May 2012, accepted 27 June 2012)
Fig. S1. (A) Far-UV CD spectra of scFvA33T1, modified scFvA33T1-Alexa 555, scFvA33 and RNase T1. (B) Differential scanning calorimetry thermogram of scFvA33T1.
Fig. S2. Flow cytometry analysis of monoclonal antibody mAbA33 binding to GPA33-positive or GPA33-negative cellular lines.
Fig. S3. Flow cytometry analysis of scFvA33T1-Alexa 555 binding to GPA33-positive SW1222 cells.
Fig. S4. Diagram showing the plasmid pPICZαAscFvA33-RNaseT1 employed to produce scFvA33T1 in P. pastoris KM71 cells.
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