Fig. S1. Thermal stability of TcdA-GT.

Fig. S2. Differential scanning calorimetry.

Fig. S3. Effect of metal addition on WT TcdA-GT thermal stability.

Fig. S4. Glucosylhydrolase activity and thermal stability of TcdA-GT.

Fig. S5. UDP–Mn2+ binding to TcdA-GT.

Fig. S6. The active site of TcdA-GT.

Fig. S7. Multiple alignment of LCT sequences.

Fig. S8. LCTs sequence identity.

Table S1. Primers used in this study.

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