Enzymatic synthesis of valerena-4,7(11)-diene by a unique sesquiterpene synthase from the valerian plant (Valeriana officinalis)
Article first published online: 25 JUL 2012
© 2012 The Authors Journal compilation © 2012 FEBS
Volume 279, Issue 17, pages 3136–3146, September 2012
How to Cite
Pyle, B. W., Tran, H. T., Pickel, B., Haslam, T. M., Gao, Z., MacNevin, G., Vederas, J. C., Kim, S.-U. and Ro, D.-K. (2012), Enzymatic synthesis of valerena-4,7(11)-diene by a unique sesquiterpene synthase from the valerian plant (Valeriana officinalis). FEBS Journal, 279: 3136–3146. doi: 10.1111/j.1742-4658.2012.08692.x
- Issue published online: 21 AUG 2012
- Article first published online: 25 JUL 2012
- Accepted manuscript online: 6 JUL 2012 12:45PM EST
- (Received 12 May 2012, revised 26 June 2012, accepted 27 June 2012)
Fig. S1. Sequence alignment of deduced amino acid sequences of VoTPS1 and VoTPS2.
Fig. S2. Semiquantitative RT-PCR analysis of the VoTPS1 and VoTPS2 transcripts in V. officinalis root and leaf.
Fig. S3. GC-MS analysis of VoTPS1 products and the terpene standards synthesized by tomato germacrene B/germacrene C synthase.
Fig. S4. SDS/PAGE gel of the purified VoTPS1 and VoTPS2 recombinant enzymes.
Table S1. GC-MS analysis of terpenoids synthesized by VoTPS1 and VoTPS2.
Table S2. Comparison of the 13C-NMR signals from the purified peak 4 with published data.
Doc. S1. Supplementary method. Chemical synthesis of valerena-4,7(11)-diene from valerenic acid.
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