Although a number of questionnaires have been developed to measure romantic jealousy, there is a lack of independent research that has substantiated their psychometric properties and minimal research that has tested and applied these measures in a consistent and reliable way. We aimed to address these gaps by providing the first validation study of an existing measure of romantic jealousy—the Multidimensional Jealousy Scale (MJS). The MJS was subjected to exploratory and confirmatory factor analyses in two separate samples of individuals in romantic relationships of at least 2 months duration. A 17-item Short-Form MJS (SF-MJS) was supported, with three factors representing cognitive, emotional, and behavioural jealousy. Internal reliabilities were high. Consistent evidence supported the discriminant and concurrent validity of the SF-MJS. Overall, the current research provides a brief, validated measure of romantic jealousy for use in the general population. Potential clinical uses are discussed.