Protein Biomarker Analysis of Primary Peyronie's Disease Cells

Authors

  • Ling X. De Young MD, MSc,

    Corresponding author
    1. Lawson Health Research Institute, Urology, London, ON, Canada;
    2. Division of Urology, St. Joseph's Health Care, London, ON, Canada;
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  • Anthony J. Bella MD,

    1. Department of Surgery, Division of Urology, University of Ottawa, Ottawa, Canada;
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  • David B. O'Gorman MSc, PhD,

    1. Lawson Health Research Institute, Urology, London, ON, Canada;
    2. Department of Surgery, The University of Western Ontario, London, ON, Canada;
    3. Plastic Surgery, Cell and Molecular Biology Laboratory of the Hand and Upper Limb Centre, London, ON, Canada;
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  • Bing S. Gan MD, PhD,

    1. Lawson Health Research Institute, Urology, London, ON, Canada;
    2. Department of Surgery, The University of Western Ontario, London, ON, Canada;
    3. Plastic Surgery, Cell and Molecular Biology Laboratory of the Hand and Upper Limb Centre, London, ON, Canada;
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  • Kok B. Lim MD,

    1. Department of Urology, Singapore General Hospital, Singapore
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  • Gerald B. Brock MD

    1. Lawson Health Research Institute, Urology, London, ON, Canada;
    2. Division of Urology, St. Joseph's Health Care, London, ON, Canada;
    3. Department of Surgery, The University of Western Ontario, London, ON, Canada;
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Ling X. De Young, MD, MSc, Urology (H311), Lawson Research Institute, 268 Grosvenor Street, London, ON, Canada N6A 4V2. Tel: 519-646-6000 x64396; Fax: 519-646-6110; E-mail: ldeyoung@lawsonimaging.ca

ABSTRACT

Introduction.  The molecular pathogenesis of Peyronie's Disease (PD) remains unclear more than 250 years after its initial description. Because of this, no test is currently available to accurately predict PD progression among those affected.

Aim.  To investigate the expression of wound healing and fibrosis-associated proteins in primary cell cultures of PD fibroblasts to determine whether altered protein expression patterns can be used as predictors of clinical course and natural history.

Methods.  Primary cell cultures derived from normal Tunica albuginea tissue and PD plaque tissue were examined by immuno-cytochemistry. Protein expression profiles were analyzed by Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS) and Western immunoblotting.

Main Outcome Measures.  Expression of wound healing and fibrosis-associated proteins and protein expression patterns were assessed.

Results.  Statistically significant increases in smooth muscle α-actin, β-catenin, and Heat shock proteins (Hsp47) were identified in cells derived from PD relative to cells derived from normal Tunica albuginea tissue. Changes in TGFβ-1 receptor and Fibronectin were also observed. In addition, altered expression of additional as yet unidentified proteins at 4.7, 8.9, 10.8, 16.8, and 76.8 kDa were detected by complementary SELDI-TOF-MS approaches.

Conclusions.  Primary cells derived from PD plaques display up-regulated expression of several proteins that are established components of fibrosis and wound healing. In addition, changes in other, as yet unidentified proteins were measured. It will be of interest to conduct further studies to see whether these dysregulated protein peaks represent potential biological markers of disease progression. De Young LX, Bella AJ, O'Gorman DB, Gan BS, Lim KB, and BrockGB. Protein biomarker analysis of primary Peyronie's disease cells. J Sex Med 2010;7:99–106.

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