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A New Perfusion Model for Studying Erectile Function

Authors

  • Chen Zhao MD,

    1. Department of Urology, Medical School, and Institute for Medical Sciences, Chonbuk National University, and Research Institute of Clinical Medicine and CTC for Medical Device of Chonbuk National University Hospital, Jeonju 561-712, Korea;
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  • Han Jung Chae PhD,

    1. Department of Pharmacology, Medical School, Chonbuk National University, Jeonbuk, Korea;
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  • Suhn Hee Kim MD, PhD,

    1. Department of Physiology, Medical School, Chonbuk National Unviersity, Jeonju, Jeonbuk, Korea;
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  • Wan Shou Cui MD, PhD,

    1. Andrology Center, Peking University First Hospital, Peking, China;
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  • Sung Won Lee MD, PhD,

    1. Department of Urology, Medical School, Sungkyunkwan University, Seoul, Korea;
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  • Ju-Hong Jeon PhD,

    1. Department of Physiology, Seoul National University Medical School, Seoul, Korea
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  • Jong Kwan Park MD, PhD

    Corresponding author
    1. Department of Urology, Medical School, and Institute for Medical Sciences, Chonbuk National University, and Research Institute of Clinical Medicine and CTC for Medical Device of Chonbuk National University Hospital, Jeonju 561-712, Korea;
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Jong Kwan Park, MD, PhD, Urology, School of Medicine, Chonbuk National University San 2-20, Keumam-dong, Jeonju, South Korea. Tel: 82-63-250-1510; Fax: 82-63-250-1564; E-mail: rain@chonbuk.ac.kr

ABSTRACT

Introduction.  The present study introduces a newly devised in vitro penile perfusion model, which allows the measurement of changes in intracavernosum pressure (ICP), tension, and cyclic nucleotides in penile tissue in a one-step procedure.

Aims.  We compared the penile perfusion model with the classical penile strip chamber model to develop the new model.

Methods.  We investigated the role of nitric oxide-cyclic guanosine monophosphate pathway in both systems activated by acetylcholine or electrical field stimulation (EFS). We measured cGMP (cyclic guanosine monophosphate) in the penile corpus cavernosum smooth muscle in both systems and perfusates in the penile perfusion model. We determined ICP, tension, and cGMP simultaneously in penile perfusion model during the experiments.

Main Outcome Measures.  Changes of tension and cGMP in both models, and of cGMP in perfusates and of ICP in penile perfusion model.

Results.  In both systems, acetylcholine relaxed the phenylephrine-precontracted penile corpus cavernosum smooth muscle in a concentration-dependent manner. EFS decreased the tension in both models and ICP in the penile perfusion model in a frequency-dependent manner. Pretreatment with atropine, Nω nitro-L-arginine-methyl ester (L-NAME), and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) blocked the acetylcholine-induced decrease in the tension in both models and ICP in penile perfusion model. Acetylcholine increased cGMP levels in the perfusate of penile perfusion model and the changes were attenuated by tetrodotoxin (TTX), atropine, L-NAME and ODQ. EFS increased cGMP levels of the penile corpus cavernosum smooth muscle of both models and the changes were attenuated by TTX, atropine, L-NAME, and ODQ.

Conclusion.  The results indicated that the responses of penile strip and perfused penile tissue to acetylcholine and EFS were very similar in both models in terms of tension and cGMP levels. Furthermore, the new model has more advantages over the classical penile strip chamber model in measuring intracavernosum metabolic changes and ICP without interruption of the procedure. Zhao C, Chae HJ, Kim SH, Cui WS, Lee SW, Jeon J-H, and Park JK. A new perfusion model for studying erectile function. J Sex Med 2010;7:1419–1428.

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