Cryopreserved Penile Tunica Albuginea for Allotransplantation: A Morphological and Ultrastructural Investigation
Article first published online: 22 MAY 2012
© 2012 International Society for Sexual Medicine
The Journal of Sexual Medicine
Volume 9, Issue 9, pages 2378–2388, September 2012
How to Cite
Loreto, C., Orlandi, A., Ferlosio, A., Djinovic, R., Basic, D., Bettocchi, C., Rutigliano, M., Barbagli, G., Vespasiani, G., Caltabiano, R., Musumeci, G. and Sansalone, S. (2012), Cryopreserved Penile Tunica Albuginea for Allotransplantation: A Morphological and Ultrastructural Investigation. Journal of Sexual Medicine, 9: 2378–2388. doi: 10.1111/j.1743-6109.2012.02789.x
- Issue published online: 4 SEP 2012
- Article first published online: 22 MAY 2012
- Cryofixation Methods;
- Penile Tunica Albuginea;
- Peyronie's Disease
Introduction. Peyronie's disease, a connective tissue disorder of penile tunica albuginea (TA) associated with penile deformity, curvature, pain, and erectile dysfunction, is best managed surgically, but suitable graft biomaterials are not available.
Aim. To establish whether cryopreservation affects human TA in view of its use in allotransplants.
Methods. The effects on TA samples of the two most widely used tissue cryopreservation methods were investigated using an ad hoc panel of histochemical, immunohistochemical, and ultrastructural tests. Apoptotic cells were evaluated using the terminal deoxynucleotidyl transferase method of end labeling (TUNEL) assay.
Main Outcome Measures. Assessment of tissue integrity and arrangement of collagen and elastic fibers in thawed TA.
Results. Both cryofixation methods provided TA tissue suitable for use as graft material. Significant ultrastructural changes, namely, a greater diameter of collagen fibrils, were detected in sections preserved in liquid nitrogen; nonetheless, such increase never exceeded the normal range. The comprehensive panel of assays used proved suitable to characterize the thawed tissue.
Conclusion. Human TA is suitable for cryopreservation; freezing at −80°C provides better results than preservation in liquid nitrogen. Loreto C, Orlandi A, Ferlosio A, Djinovic R, Basic D, Bettocchi C, Rutigliano M, Barbagli G, Vespasiani G, Caltabiano R, Musumeci G, and Sansalone S. Cryopreserved penile tunica albuginea for allotransplantation: A morphological and ultrastructural investigation. J Sex Med 2012;9:2378–2388.