Differential expression of miR-21, miR-125b and miR-191 in breast cancer tissue
Article first published online: 23 JUL 2012
© 2012 Wiley Publishing Asia Pty Ltd
Asia-Pacific Journal of Clinical Oncology
Volume 9, Issue 1, pages 53–59, March 2013
How to Cite
MAR-AGUILAR, F., LUNA-AGUIRRE, C. M., MORENO-ROCHA, J. C., ARAIZA-CHÁVEZ, J., TREVINO, V., RODRÍGUEZ-PADILLA, C. and RESÉNDEZ-PÉREZ, D. (2013), Differential expression of miR-21, miR-125b and miR-191 in breast cancer tissue. Asia-Pacific Journal of Clinical Oncology, 9: 53–59. doi: 10.1111/j.1743-7563.2012.01548.x
- Issue published online: 19 FEB 2013
- Article first published online: 23 JUL 2012
- Accepted for publication 11 March 2012.
- breast cancer;
Aims: To develop new biomarkers for early detection and to inform effective clinical management of breast cancer.
Methods: Real-time polymerase chain reaction was used to profile microRNA (miRNA) in tumor tissue from 50 breast cancer patients using non-tumor breast tissue from each patient as a control. We have focussed on three miRNA; miR-21, miR-125b and miR-191, all of which have been implicated in breast cancer with either proven or predicted target genes involved in critical cancer-associated cellular pathways.
Results: Upregulation of miR-21 and miR-191 and downregulation of miR-125b, was found in breast cancer tissue. Combined expression analysis of miR-125b/miR-191 increased sensitivity to 100% and specificity to 94% while miR-21/miR-191 increased to 92% and 100%, respectively. Therefore, combination of two miRNA gives a better prediction than individual miRNA.
Conclusions: We could differentiate between breast cancer and adjacent non-tumor breast tissue as a control with a high degree of sensitivity and specificity in the Mexican population using a combined expression analysis of only two miRNA. These observations, although a proof of principle finding at this time, show that a combined expression profile of two miRNA (miR-125b/miR-191 and miR-21/miR-191) can discriminate between breast cancer and non-tumor tissue with high specificity and sensitivity.