SNPs in human β-defensin 1 gene (DEFB1): frequencies in a Mexican population and new PCR–RFLPs assays

Authors

  • E. Prado-Montes de Oca,

    1.  PhD Program in Human Genetics, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, México,
    2. Genetics Division, Centro de Investigación Biomédica de Occidente (CIBO-IMSS), Guadalajara, Jalisco, México,
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  • H. Rangel-Villalobos,

    1.  PhD Program in Human Genetics, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, México,
    2. Molecular Genetics Laboratory, Centro Universitario de la Ciénega, Universidad de Guadalajara (CUCI-UdeG), Ocotlán, Jalisco, México and
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  • M. P. Gallegos-Arreola,

    1.  PhD Program in Human Genetics, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, México,
    2. Molecular Medicine Division; Centro de Investigación Biomédica de Occidente (CIBO-IMSS), Guadalajara, Jalisco, México
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  • L. Sandoval,

    1.  PhD Program in Human Genetics, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, México,
    2. Genetics Division, Centro de Investigación Biomédica de Occidente (CIBO-IMSS), Guadalajara, Jalisco, México,
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  • L. E. Figuera

    1.  PhD Program in Human Genetics, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, México,
    2. Genetics Division, Centro de Investigación Biomédica de Occidente (CIBO-IMSS), Guadalajara, Jalisco, México,
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Dr Luis E. Figuera, División de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia SL, 44340 Guadalajara, Jalisco, México. Tel: (+52-33) 3668-3000, ext. 31929; Fax: (+52-33) 3618-1756; E-mail: lef@koch.mb.udg.mx

Summary

Polymerase chain reaction–restriction fragment length polymorphism assays for two single nucleotide polymorphisms in the human β-defensin 1 gene have been validated with real-time PCR in 101 healthy individuals from western Mexico. Allele frequencies were 52.5% (692-A) and 98.5% (1836-A). These assays can be confidently used as a cheaper alternative genotyping method for these sites.

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