In grassland in the vicinity of Kharkiv, Ukraine, we determined the floristic composition of the plant communities, v-values of the species and above-ground plant biomass. The plant communities in Kharkiv were dominated by Stipa capillata L., Medicago romanica Prodan, Inula ensifolia L. and Poa angustifolia L. There were 46 species in eight 1-m2 quadrates and the above-ground plant biomass was 245 g m−2 in the Stipa capillata stand. In the Medicago romanica stand, the number of species and above-ground plant biomass were 37 per 10 quadrates of 1 m2 and 195 g m−2, respectively. The numbers of species in the stands were 40 in Poa angustifolia and 45 in Inula ensifolia. The plant biomass of the Poa angustifoia stand was 380 g m−2. These values were similar to those from Inner Mongolia. The species diversity of the stand (H) was calculated as: H = −Σpi log2 pi. The species diversity of the two stands was 2.98 for the Stipa capillata stand and 2.66 in the Medicago romanica stand. The stand changed by various human impacts, such as grazing and cultivation. Plantago schwarzenbergiana Schur. seemed to become established after the exposure of Stipa capillata stands to grazing, while Inula ensifolia stands became established in an abandoned field. The species composition in Kharkiv was different from that of Inner Mongolia, though the genus of plants was similar.