A comparison of some quantitative methods of extracting small vermiform nematodes from soil
Article first published online: 28 JUN 2008
Annals of Applied Biology
Volume 55, Issue 1, pages 25–38, February 1965
How to Cite
WHITEHEAD, A. G. and HEMMING, J. R. (1965), A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55: 25–38. doi: 10.1111/j.1744-7348.1965.tb07864.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received 27 August 1964
When 200 ml. dispersed soil is sedimented from an obliquely rising water current in a simple compartmented tank about three-quarters of the nematodes are extracted. About 95% of the nematodes in the concentrated suspension can be separated finally from mineral and heavy organic particles by centrifugal notation. When mobile nematodes were finally separated from soil particles by paper tissue, this sedimentation method extracted as many nematodes from sand and loam as Seinhorst's two-flask and elutriation methods, but in one test extracted fewer Tylenchorhynchus from clay and in another fewer Paratylenchus from clay than the elutriation method. The method is quicker (4 or 6 instead of 30–45 min.) and easier.
Mobile nematodes can be extracted from 300 ml. soil spread out on paper tissue in 23. 33 cm. trays of 8 mesh/cm. phosphor-bronze gauze, just resting on shallow water. The suspension obtained after 24 hr. at 16–18°C. was concentrated to 10–15 ml. without loss by sedimentation in two tapered cylinders, one of 8 cm. bore, the other of 2.6 cm. bore. This method usually extracted significantly more nematodes than the sedimentation, two-flask and elutriation methods.