A comparison of methods for inoculating bean plants with Elsinoë phaseoli and some factors affecting infection
Article first published online: 26 FEB 2008
Annals of Applied Biology
Volume 125, Issue 1, pages 97–104, August 1994
How to Cite
PHILLIPS, A. J. L. (1994), A comparison of methods for inoculating bean plants with Elsinoë phaseoli and some factors affecting infection. Annals of Applied Biology, 125: 97–104. doi: 10.1111/j.1744-7348.1994.tb04950.x
- Issue published online: 26 FEB 2008
- Article first published online: 26 FEB 2008
- Accepted 25 May 1994, Received 1 December 1993
- Phaseolus vulgaris;
Bean (Phaseolus vulgaris) leaves were inoculated with a conidial suspension of Elsinoe phaseoli by spraying, by applying a 10 μl drop, by a cotton wool technique or by using a camel hair brush. Spray application resulted in a uniform distribution of lesions over the leaf surface and gave consistent levels of infection between repetitions. Drop application allowed good control of the amount of inoculum applied. Although brush or cotton wool techniques gave high levels of infection it was not possible to control the amount of inoculum. Only immature tissues were susceptible to infection.
Five cultivars were inoculated with two isolates and disease was scored on the basis of numbers of lesions and lesion type (scab severity). The small white cvs PAN 181 and Teebus were the most resistant; lesions were small and generally fewer than on the large-seeded red speckled sugar bean cvs Umlazi and Umvoti. The value of the different inoculation techniques in studies on bean scab is discussed.