Development of a dot immunobinding assay to detect Phytophthora spp. in naturally dark-rooted woody plants

Authors

  • RENATE HAHN,

    1. Institute for Plant Diseases and Plant Protection, University of Hannover, Herrenhäserstrasse 2, 30419 Hannover, Germany
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  • SABINE WERRES

    Corresponding author
    1. Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Protection in Horticultural Crops, Messeweg 11/12, 38104 Braunschweign, Germany
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    • 3

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Summary

An indirect dot immunobinding assay (DIBA) with a PAb raised against an isolate of P. cinnumomi was evaluated to detect Phytophthora spp. in naturally dark-rooted woody plants. Screening of different modifications of the procedure were done with root samples of Chamaecyparis lawsoniana non-infected and infected with P. cinnamomi. With the optimised DIBA procedure, root infection could be diagnosed by a clearly defined halo around the spot and by a colour change in the spot using Fast Red or Fast Blue substrate. Detection of different Phytophthora species in Chamaecyparis plants from commercial nurseries was successful with the optimised procedure.

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