Supported by the National Priority Basic Research Programs of People's Republic of China: Biosafety Study on GMOs of Agricultural Importance (001CB10902 to L-JQ), the National Natural Science Foundation of China (30470358), and the Excellent Young Teachers Program of MOE, China (to L-JQ).
Overexpression of the Wounding-Responsive Gene AtMYB15 Activates the Shikimate Pathway in Arabidopsis
Version of Record online: 2 OCT 2006
Journal of Integrative Plant Biology
Volume 48, Issue 9, pages 1084–1095, September 2006
How to Cite
Chen, Y., Zhang, X., Wu, W., Chen, Z., Gu, H. and Qu, L.-J. (2006), Overexpression of the Wounding-Responsive Gene AtMYB15 Activates the Shikimate Pathway in Arabidopsis. Journal of Integrative Plant Biology, 48: 1084–1095. doi: 10.1111/j.1744-7909.2006.00311.x
- Issue online: 2 OCT 2006
- Version of Record online: 2 OCT 2006
- Received 19 Jun. 2006 Accepted 3 Jul. 2006
- MYB transcription factor;
- shikimate pathway;
- transcriptional activation domain;
- wounding inducible
The MYB transcription factor genes play important roles in many developmental processes and various defense responses of plants. The shikimate pathway is a major biosynthetic pathway for the production of three aromatic amino acids and other aromatic compounds that are involved in multiple responses of plants, including protection against UV and defense. Herein, we describe the characterization of the R2R3-MYB gene AtMYB15 as an activator of the shikimate pathway in Arabidopsis. The AtMYB15 protein is nuclear localized and a transcriptional activation domain is found in its C-terminal portion. Northern blots showed that AtMYB15 is an early wounding-inducible gene. Resutls of microarray analysis, confirmed using quantitative real-time polymerase chain reaction, showed that overexpression of AtMYB15 in transgenic plants resulted in elevated expression of almost all the genes involved in the shikimate pathway. Bioinformatics analysis showed that one or more AtMYB15-binding AC elements were detected in the promoters of these upregulated genes. Furthermore, these genes in the shikimate pathway were also found to be induced by wounding. These data suggest an important role of AtMYB15 as a possible direct regulator of the Arabidopsis shikimate pathway in response to wounding.
(Managing editor: Ya-Qin Han)