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Determination of n+1 Gamete Transmission Rate of Trisomics and Location of Gene Controlling 2n Gamete Formation in Chinese Cabbage (Brassica rapa)
Article first published online: 12 NOV 2008
DOI: 10.1111/j.1744-7909.2008.00765.x
© 2008 Institute of Botany, the Chinese Academy of Sciences
Additional Information
How to Cite
Zhang, C.-H., Li, X.-F., Shen, S.-X., Yuan, H. and Xuan, S.-X. (2009), Determination of n+1 Gamete Transmission Rate of Trisomics and Location of Gene Controlling 2n Gamete Formation in Chinese Cabbage (Brassica rapa). Journal of Integrative Plant Biology, 51: 29–34. doi: 10.1111/j.1744-7909.2008.00765.x
Publication History
- Issue published online: 29 DEC 2008
- Article first published online: 12 NOV 2008
- Received 29 Dec. 2007 Accepted 9 Jun. 2008
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Keywords:
- Chinese cabbage;
- gene location;
- n+1 gamete transmission;
- primary trisomics;
- 2n gametes
Abstract
A set of trisomics of Chinese cabbage was used for determining the n+1 gamete transmission rate and locating the gene controlling 2n gamete formation on the corresponding chromosome. The results showed that the transmission rates of extra chromosomes in different trisomics varied from 0% to 15.38% by male gametes and from 0% to 17.39% by female gametes. Of the nine F2 populations derived from the hybridizations between each trisomic and Bp058 (2n gamete material), only Tri-4×Bp058 showed that the segregation ratio of plants without 2n gamete formation to plants with 2n gamete formation was 10.38:1, which fitted the expected segregation ratio of the trisomics (AAa) based on the 7.37% of n+1 gamete transmission through female and 5.88% through male. In other populations the segregation ratios varied from 2.48:1 to 3.72:1, which fitted the expected 3:1 segregation ratio of the bisomics (Aa). These results suggested that the gene controlling 2n gamete formation in Chinese cabbage Bp058 was located on chromosome 4. Further trisomic analysis based on the chromosome segregation and the incomplete stochastic chromatid segregation indicated that the gene locus was tightly linked to the centromere.