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Molecular Characterization of Two Silenced y-type Genes for Glu-B1 in Triticum aestivum ssp. yunnanese and ssp. tibetanum
Article first published online: 27 NOV 2008
DOI: 10.1111/j.1744-7909.2008.00775.x
© 2008 Institute of Botany, the Chinese Academy of Sciences
Additional Information
How to Cite
Yuan, Z.-W., Chen, Q.-J., Zhang, L.-Q., Yan, Z.-H., Zheng, Y.-L. and Liu, D.-C. (2009), Molecular Characterization of Two Silenced y-type Genes for Glu-B1 in Triticum aestivum ssp. yunnanese and ssp. tibetanum. Journal of Integrative Plant Biology, 51: 93–99. doi: 10.1111/j.1744-7909.2008.00775.x
Publication History
- Issue published online: 29 DEC 2008
- Article first published online: 27 NOV 2008
- Received 26 Jan. 2008 Accepted 17 Jun. 2008
- Abstract
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Keywords:
- 1By;
- common wheat;
- gene silence;
- Glu-B1 locus;
- high molecular weight glutenin subunits
Abstract
The high molecular weight glutenin subunits (HMW-GSs) are a major class of common wheat storage proteins. The bread-making quality of common wheat flour is influenced by the composition of HMW-GSs. In the present study, two unexpressed 1By genes from Triticum aesitvum L.ssp.yunnanese AS332 and T. aesitvum ssp.tibetanum AS908 were respectively cloned and characterized. The results indicated that both of the silenced 1By genes in AS332 and AS908 were 1By9. In contrast to previously reported mechanisms for silenced genes 1Ax and 1Ay, which was due to the insertion of transposon elements or the presence of premature stop codon via base substitution of C→T transition in trinucleotides CAA or CAG, the silence of 1By9 genes was caused by premature stop codons via the deletion of base A in trinucleotide CAA, which lead to frameshift mutation and indirectly produced several premature stop codons (TAG) downstream of the coding sequence.