Supported by the State Key Basic Research and Development Plan of China (2003CB114302) and the National Natural Science Foundation of China (30571122).
Alternative Splicing and Differential Expression of Two Transcripts of Nicotine Adenine Dinucleotide Phosphate Oxidase B Gene from Zea mays
Article first published online: 1 MAR 2009
© 2009 Institute of Botany, the Chinese Academy of Sciences
Journal of Integrative Plant Biology
Volume 51, Issue 3, pages 287–298, March 2009
How to Cite
Lin, F., Zhang, Y. and Jiang, M.-Y. (2009), Alternative Splicing and Differential Expression of Two Transcripts of Nicotine Adenine Dinucleotide Phosphate Oxidase B Gene from Zea mays. Journal of Integrative Plant Biology, 51: 287–298. doi: 10.1111/j.1744-7909.2008.00808.x
- Issue published online: 1 MAR 2009
- Article first published online: 1 MAR 2009
- Received 18 Jun. 2008 Accepted 9 Nov. 2008
- abiotic stress;
- alternative splicing;
- respiratory burst oxidase homolog;
- Zea mays
With the exception of rice, little is known about the existence of respiratory burst oxidase homolog (rboh) gene in cereals. The present study reports the cloning and analysis of a novel rboh gene, termed ZmrbohB, from maize (Zea mays L.). The full-length cDNA of ZmrbohB encodes a 942 amino acid protein containing all of the respiratory burst oxidase homolog catalytically critical motifs. Alternative splicing of ZmrbohB has generated two transcript isoforms, ZmrbohB-α and -β. Spliced transcript ZmrbohB-β retains an unspliced intron 11 that carries a premature termination codon and probably leads to nonsense-mediated mRNA decay. Expression analysis showed that two splice isoforms were differentially expressed in various tissues and at different developmental stages, and the major product was ZmrbohB-α. The transcripts of ZmrbohB-α accumulated markedly when the maize seedlings were subjected to various abiotic stimuli, such as wounding, cold (4 °C), heat (40 °C), UV and salinity stress. In addition, several abiotic stimuli also affected the alternative splicing pattern of ZmrbohB except wounding. These results provide new insight into roles in the expression regulation of plant rboh genes and suggest that ZmrbohB gene may play a role in response to environmental stresses.