These authors contributed equally to this work.
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Comparative Proteomic Analysis of Arabidopsis Mature Pollen and Germinated Pollen
Article first published online: 30 MAR 2009
DOI: 10.1111/j.1744-7909.2009.00823.x
© 2009 Institute of Botany, the Chinese Academy of Sciences
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How to Cite
Zou, J., Song, L., Zhang, W., Wang, Y., Ruan, S. and Wu, W.-H. (2009), Comparative Proteomic Analysis of Arabidopsis Mature Pollen and Germinated Pollen. Journal of Integrative Plant Biology, 51: 438–455. doi: 10.1111/j.1744-7909.2009.00823.x
Publication History
- Issue published online: 23 APR 2009
- Article first published online: 30 MAR 2009
- Received 12 Nov. 2008 Accepted 9 Feb. 2009
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Keywords:
- 2D-polyacrylamide gel electrophoresis;
- Arabidopsis thaliana;
- matrix-assisted laser desorption ionization time-of-flight mass spectrometry;
- pollen;
- pollen tube;
- proteome
Abstract
Proteomic analysis was applied to generating the map of Arabidopsis mature pollen proteins and analyzing the differentially expressed proteins that are potentially involved in the regulation of Arabidopsis pollen germination. By applying 2-D electrophoresis and silver staining, we resolved 499 and 494 protein spots from protein samples extracted from pollen grains and pollen tubes, respectively. Using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, we identified 189 distinct proteins from 213 protein spots expressed in mature pollen or pollen tubes, and 75 new identified proteins that had not been reported before in research into the Arabidopsis pollen proteome. Comparative analysis revealed that 40 protein spots exhibit reproducible significant changes between mature pollen and pollen tubes. And 21 proteins from 17 downregulated and six upregulated protein spots were identified. Functional category analysis indicated that these differentially expressed proteins mainly involved in signaling, cellular structure, transport, defense/stress responses, transcription, metabolism, and energy production. The patterns of changes at protein level suggested the important roles for energy metabolism-related proteins in pollen tube growth, accompanied by the activation of the stress response pathway and modifications to the cell wall.