Endogenous Hydrogen Peroxide Plays a Positive Role in the Upregulation of Heme Oxygenase and Acclimation to Oxidative Stress in Wheat Seedling Leaves

Authors

  • Xiao-Yue Chen,

    1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
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  • Xiao Ding,

    1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
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  • Sheng Xu,

    1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
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  • Ren Wang,

    1. Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences (Mem. Sun Yat-Sen), Jiangsu Province Key Laboratory for Plant Ex-situ Conservation, Nanjing 210014, China
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  • Wei Xuan,

    1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
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  • Ze-Yu Cao,

    1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
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  • Jian Chen,

    1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
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  • Hong-Hong Wu,

    1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
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  • Mao-Bing Ye,

    1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
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  • Wen-Biao Shen

    Corresponding author
    1. College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
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  • Supported by the fund for Creative Experimental Project of Undergraduate Students in Jiangsu Province of China (96), the program for New Century Excellent Talents in University (NCET-07-0441), the Natural Science Foundation of China (30671248), and the grant obtained from the 111 Project of China (B07030).

*Author for correspondence.
Tel.: +86 25 8439 8602;
Fax: +86 25 8439 6542;
E-mail: <wbshenh@njau.edu.cn>.

Abstract

Pretreatment of lower H2O2 doses (0.05, 0.5 and 5 mM) for 24 h was able to dose-dependently attenuate lipid peroxidation in wheat seedling leaves mediated by further oxidative damage elicited by higher dose of H2O2 (150 mM) for 6 h, with 0.5 mM H2O2 being the most effective concentrations. Further results illustrated that 0.5 mM H2O2 pretreatment triggered the biphasic production of H2O2 during a 24 h period. We also noticed that only peak I (0.25 h) rather than peak II (4 h) was approximately consistent with the enhancement of heme oxygenase (HO) activity, HO-1 gene expression. Meanwhile, enhanced superoxide dismutase (SOD) activity, Mn-SOD and Cu,Zn-SOD transcripts might be a potential source of peak I of endogenous H2O2. Further results confirmed that 0.5 mM H2O2 treatment for 0.5 h was able to upregulate HO gene expression, which was detected by enzyme activity determination, semi-quantitative reverse transcription-polymerase chain reaction and western blotting. Meanwhile, the application of N,N’-dimethylthiourea, a trap for endogenous H2O2, not only blocked the upregulation of HO, but also reversed the corresponding oxidation attenuation. Together, the above results suggest that endogenous H2O2 production (peak I) plays a positive role in the induction of HO by enhancing its mRNA level and protein expression, thus leading to the acclimation to oxidative stress.

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