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Research Article
Evaluation of Protein Extraction Methods for Vitis vinifera Leaf and Root Proteome Analysis by Two-Dimensional Electrophoresis
Article first published online: 16 JUN 2010
DOI: 10.1111/j.1744-7909.2010.00973.x
© 2010 Institute of Botany, Chinese Academy of Sciences
Additional Information
How to Cite
Jellouli, N., Salem, A. B., Ghorbel, A. and Jouira, H. B. (2010), Evaluation of Protein Extraction Methods for Vitis vinifera Leaf and Root Proteome Analysis by Two-Dimensional Electrophoresis. Journal of Integrative Plant Biology, 52: 933–940. doi: 10.1111/j.1744-7909.2010.00973.x
Publication History
- Issue published online: 16 JUN 2010
- Article first published online: 16 JUN 2010
- Received 8 Dec. 2009 Accepted 27 May 2010
- Abstract
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Abstract
An efficient protein extraction method is crucial to ensure successful separation by two-dimensional electrophoresis (2-DE) for recalcitrant plant species, in particular for grapevine (Vitis vinifera L.). Trichloroacetic acid-acetone (TCA-acetone) and phenol extraction methods were evaluated for proteome analysis of leaves and roots from the Tunisian cultivar ‘Razegui’. The phenol-based protocol proved to give a higher protein yield, a greater spot resolution, and a minimal streaking on 2-DE gels for both leaf and root tissues compared with the TCA-based protocol. Furthermore, the highest numbers of detected proteins on 2-DE gels were observed using the phenol extraction from leaves and roots as compared with TCA-acetone extraction.