LHD1, an Allele of DTH8/Ghd8, Controls Late Heading Date in Common Wild Rice (Oryza rufipogon)

Authors

  • Xiaodong Dai,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Younian Ding,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Lubin Tan,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Yongcai Fu,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Fengxia Liu,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Zuofeng Zhu,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Xianyou Sun,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Xuewen Sun,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Ping Gu,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Hongwei Cai,

    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author
  • Chuanqing Sun

    Corresponding author
    1. State Key Laboratory of Plant Physiology and Biochemistry, National Evaluation Centre for Agricultural Wild Plants (Rice), Laboratory of Crop Heterosis and Utilization of the Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
    Search for more papers by this author

Tel/Fax: +86 10 6273 1811; E-mail: suncq@cau.edu.cn

Abstract

Flowering at suitable time is very important for plants to adapt to complicated environments and produce their seeds successfully for reproduction. In rice (Oryza rufipogon Griff.) photoperiod regulation is one of the important factors for controlling heading date. Common wild rice, the ancestor of cultivated rice, exhibits a late heading date and a more sensitive photoperiodic response than cultivated rice. Here, through map-based cloning, we identified a major quantitative trait loci (QTL) LHD1 (Late Heading Date 1), an allele of DTH8/Ghd8, which controls the late heading date of wild rice and encodes a putative HAP3/NF-YB/CBF-A subunit of the CCAAT-box-binding transcription factor. Sequence analysis revealed that several variants in the coding region of LHD1 were correlated with a late heading date, and a further complementary study successfully rescued the phenotype. These results suggest that a functional site for LHD1 could be among those variants present in the coding region. We also found that LHD1 could down-regulate the expression of several floral transition activators such as Ehd1, Hd3a and RFT1 under long-day conditions, but not under short-day conditions. This indicates that LHD1 may delay flowering by repressing the expression of Ehd1, Hd3a and RFT1 under long-day conditions.

Ancillary