Extracorporeal Photopheresis Normalizes Some Lymphocyte Subsets (Including T Regulatory Cells) in Chronic Graft-Versus-Host-Disease
Version of Record online: 5 AUG 2008
© 2008 The Authors. Journal compilation © 2008 International Society for Apheresis
Therapeutic Apheresis and Dialysis
Volume 12, Issue 4, pages 311–318, August 2008
How to Cite
Bladon, J. and Taylor, P. (2008), Extracorporeal Photopheresis Normalizes Some Lymphocyte Subsets (Including T Regulatory Cells) in Chronic Graft-Versus-Host-Disease. Therapeutic Apheresis and Dialysis, 12: 311–318. doi: 10.1111/j.1744-9987.2008.00593.x
- Issue online: 5 AUG 2008
- Version of Record online: 5 AUG 2008
- Received September 2007; revised October 2007.
- Extracorporeal photopheresis;
- Flow cytometry;
- T regulatory cell
Abstract: Extracorporeal photopheresis (ECP) has been demonstrated to be clinically effective for the treatment of steroid-refractory graft-versus-host-disease (GvHD). Recently, a murine model suggested that ECP may modulate the allo-reactivity, seen in GvHD, by enhancement of T regulatory cells. T regulatory cells are an important component of immune tolerance and reduced levels of them have been observed in chronic GvHD. Samples were taken from eight chronic GvHD patients prior to receiving any ECP therapy and after three months of ECP. Samples were also obtained from eight age- and sex-matched normal controls. Each sample was evaluated for absolute levels of total lymphocytes and CD4+ and CD8+ T cells. T regulatory cell numbers were also assessed accordingly: CD4+CD25high, CD4+CD25highCD69-, CD4+CD25highCD152+ and CD4+CD25highFOXP3+. Following three months of ECP, increases in the absolute counts of total lymphocytes, CD4+ and CD8+ T cells and T regulatory cells were observed; however, no selective statistical increase in the percentage of T regulatory cell numbers was observed within the CD4+ T cell compartment. ECP induces an increase in T regulatory cell numbers; however, this is not specific as there is also an enhancement of both total lymphocyte and CD4+ T cell numbers. The positive effect of ECP may therefore depend on a more generic re-adjustment of immune homeostasis.