Presented in part at the 29th Annual Meeting of the Japanese Society for Apheresis held November 21–23, 2008, in Hiroshima, Japan.
Biological Effect of Anaphylatoxin C5a on the Generation of Anti-inflammatory Substances in Leukocyte Adsorption
Article first published online: 26 NOV 2009
© 2009 The Authors. Journal compilation © 2009 International Society for Apheresis
Therapeutic Apheresis and Dialysis
Volume 13, Issue 6, pages 509–514, December 2009
How to Cite
Nishise, S., Takeda, Y., Nishise, Y., Fujishima, S., Orii, T., Otake, S., Sato, T., Sasaki, Y., Takeda, H. and Kawata, S. (2009), Biological Effect of Anaphylatoxin C5a on the Generation of Anti-inflammatory Substances in Leukocyte Adsorption. Therapeutic Apheresis and Dialysis, 13: 509–514. doi: 10.1111/j.1744-9987.2009.00779.x
- Issue published online: 26 NOV 2009
- Article first published online: 26 NOV 2009
- Received February 2009.
- Complement C5a;
- Hepatocyte growth factor;
- Interleukin-1 receptor antagonist
Anaphylatoxins, which are involved in both pro-inflammatory processes and a variety of anti-inflammatory effects, are produced during granulocyte and monocyte adsorptive apheresis. We noticed the anti-inflammatory effects of C5a, the strongest anaphylatoxin, in granulocyte and monocyte adsorptive apheresis. The aim of this study was to investigate the effect of C5a on interleukin-1 receptor antagonist (IL-1ra) and hepatocyte growth factor (HGF) generation in granulocyte and monocyte adsorption. Peripheral blood containing nafamostat mesilate as an endogenous complement activation inhibitor was divided into four groups: (1) no recombinant C5a added, no contact with cellulose acetate (CA) beads (control group); (2) no C5a added, contact with CA beads; (3) C5a added, no contact with CA beads; and (4) C5a added, contact with CA beads. After incubation, IL-1ra and HGF in plasma were measured. IL-1ra was significantly higher in group 3, in which only C5a was added in the absence of CA beads, compared to groups 2 (P < 0.01) and 4 (P < 0.05). HGF was significantly higher only in group 4, in which C5a was added in the presence of CA beads (P < 0.05), but did not increase in the absence of CA beads. C5a can directly induce IL-1ra generation without the granulocyte and monocyte adsorption stimuli to CA beads, but can synergistically induce HGF generation with the adsorption stimuli, indicating C5a has different effects on IL-1ra and HGF generation.